目录
摘要Ⅱ
关键词Ⅱ
AbstractⅢ
引言
引言1
1 材料与方法2
1.1 试验材料2
1.2 试验方法2
1.2.1 采集样品2
1.2.2 DNA提取与稀释2
1.2.3 设计引物3
1.2.4 确定PCR最适退火温度3
1.2.5 目的片段扩增4
1.2.6 目的片段测序4
1.2.7 位点分型5
1.2.8 数据分析5
2 结果与分析5
2.1 RPS6KB1基因g.36158079位点在三种猪群体中的分型结果5
2.2 RPS6KB1基因g.36158079位点多态性与三种猪产仔数关联性分析结果6
2.2.1 RPS6KB1基因g.36158079位点多态性与梅山猪产仔数关联性分析6
2.2.2 RPS6KB1基因g.36158079位点多态性与苏太猪产仔数关联性分析7
2.2.3 RPS6KB1基因g.36158079位点多态性与苏淮猪产仔数关联性分析8
3 讨论9
3.1 g.36158079位点与梅山猪、苏太猪和苏淮猪产仔数不显著相关的原因9
3.2 下一步应增加样本量进一步验证g.36158079位点的可靠性10
3.3 下一步可探索影响梅山猪、苏太猪和苏淮猪产仔数的其他基因位点10
4 结论10
致谢10
参考文献11
RPS6KB1基因g.36158079位点多态性与梅山猪、苏太猪和苏淮猪产仔数的关联性分析
摘 要
[目的]本研究旨在通过分析猪RPS6KB1基因g.36158079位点多态性与梅山猪、苏太猪和苏淮猪产仔数的关联性，以此进一步证明该位点对产仔数的作用，同时为梅山猪、苏太猪和苏淮猪产仔数分子标记辅助育种提供新的标记。[方法]对猪RPS6KB1基因g.36158079位点在梅山猪、苏太猪和苏淮猪中进行测序并分型，再利用SAS 9.2软件对该位点基因型进行与产仔数的关联性分 *51今日免费论文网|www.jxszl.com +Q: ^351916072# 
析。[结果]关联性分析结果显示，对于梅山猪、苏太猪和苏淮猪，该位点与全部胎次、经产胎次的总产仔数和产活仔数均没有显著关联，但与梅山猪全部胎次的产活仔数和苏太猪经产胎次的总产仔数之间存在关联的趋势（P < 0.1）。[结论]本试验证明猪RPS6KB1基因g.36158079位点在梅山猪、苏太猪和苏淮猪群体中具有多态性，且该位点与梅山猪全部胎次的产活仔数和苏太猪经产胎次的总产仔数之间存在关联的趋势。
CORRELATION ANALYSIS BETWEEN POLYMORPHISM OF RPS6KB1 GENE g.36158079 AND LITTER SIZE OF MEISHAN PIG, SUTAI PIG AND SUHUAI PIG
ABSTRACT
[Objective] The purpose of this study was to analyze the correlation between polymorphism of RPS6KB1 gene g.36158079 and litter size of Meishan Pig, Sutai Pig and Suhuai Pig, to further prove the effect of the locus on litter size, and to provide a new marker for molecular marker assisted breeding for litter size of Meishan pigs, Sutai pigs and Suhuai pigs. [Method] The genotypes of RPS6KB1 gene loci g.36158079 were sequenced and classified from samples of Meishan pigs, Sutai pigs and Suhuai pigs. Using SAS 9.2 software to analyze the correlation between the locus genotypes and litter size. [Results] The results of correlation analysis showed that for Meishan pigs, Sutai pigs or Suhuai pigs, there was no correlation between the locus and total number born and number born alive in all parities and later parities, but a trend toward significant correlation was found between the locus and the number born alive of all parities in Meishan pigs and the total number born of multiparities in Sutai pigs(P < 0.1). [Conclusion] The results showed that the RPS6KB1 gene g.36158079 had polymorphism in Meishan pigs, Sutai pigs and Suhuai pigs, and there was a trend toward significant correlation between the locus and the number born alive of all parities in Meishan pigs and the total number born of multiparities in Sutai pigs.

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