目录
摘要1
关键词1
Abstract2
Key words2
1 引言3
1.1 选题背景和文献综述 3
2 材料与方法4
2.1 材料与生长条件 4
2.2 方法 4
2.2.1 水稻ACS基因家族的序列提取和成员鉴定4
2.2.2水稻ACS基因家族序列比对4
2.2.3 水稻ACS基因家族启动子区顺式调控元件的分析5
2.2.4 水稻ACS基因家族激素响应元件位点预测5
3 结果5
3.1 水稻ACS基因家族的系统发育树5
3.2 水稻ACS基因家族启动子区顺式调控元件的分析6
3.3 水稻ACS基因家族激素响应元件位点预测7
4 讨论 8
4.1 水稻ACS基因家族启动子响应元件的讨论8
4.2 淹水胁迫响应中乙烯的重要作用9
4.3 淹水胁迫响应是多种激素共同作用的结果10
4.4 存在的问题及展望11
致谢11
参考文献11
水稻ACC合成酶基因家族启动子淹水响应元件分析
摘要
水稻是一种具有较强抗逆性的经济作物,但完全淹水和持续淹水的环境条件会对水稻的生长造成不可逆的伤害,导致严重的经济损失｡在淹水条件下水稻茎部和根部的乙烯浓度很快会增加到一个生理相关的激素水平。氨基环丙烷羧酸(1aminocyclopropane1carboxylic acid, ACC)是水稻乙烯生物合成的前体,对乙烯的合成具有重要的调控作用。根据之前的基因表达量数据,在水稻中仅有2个ACC合成酶基因(1aminocyclopropane1carboxylic acid synthase, ACS)OsACS1和OsACS5的基因表达水平在淹水过程中积累上升,这与水稻体内ACC合成酶基因家族的mRNA浓度变化趋势相一致。通过对水稻ACC合成酶基因家族启动子的响应元件预测发现,启动子区存在和乙稀,赤霉素,生长素等激素响应相关的响应元件,由此可以推测以上的激素途径在淹水条件下会被激活。以上结果初步揭 *51今日免费论文网|www.jxszl.com +Q: @351916072@ 
示了气体激素乙稀和生长促进型激素赤霉素和生长素参与到淹水诱导的水稻淹水胁迫的形成过程中,且相互影响并协同发挥调控作用。该研究为进一步揭示水稻对于淹水胁迫的反应机制做了铺垫,以及展现了ACC合成酶基因家族在水稻抗逆方面的重要性,为培养新型的耐涝水稻提供理论依据。
关键字 淹水条件 ;启动子 ; ACC合成酶 ; 水稻
RESPONSE ELEMENT ANALYSIS OF THE PROMOTER OF THE RICE ACC SYNTHASE GENE FAMILY UNDER THE WATERLOGGING CONDITIONS
ABSTRACT
Rice is a cash crop with strong stress resistance, but the environmental conditions of complete flooding and continuous flooding will cause irreversible damage to the growth of rice, resulting in serious economic losses. Rice stems under flooded conditions The ethylene concentration in the roots and roots will soon increase to a physiologically relevant hormone level. ACC(1Aminocyclopropane1carboxylic acid) is a precursor of ethylene biosynthesis in rice, and it plays an important role in regulating ethylene synthesis. According to the previous transcriptome data, there are only two ACC synthase genes (1aminocyclopropane1carboxylic acid synthase, ACS) in rice. The transcription level of OsACS1 and OsACS5 accumulates during flooding, which is the same as ACC mRNA condition in rice. By predicting the response elements of the promoter of the rice ACC synthase gene family, it is found that there are response elements related to hormone responses such as ethylene, gibberellin, and auxin in the promoter region, which can be speculated that the above hormone pathways are in flooded conditions Will be activated. The above results preliminarily reveal that the gas hormone ethylene and the growthpromoting hormones gibberellin and auxin are involved in the formation of floodinginduced flooding stress in rice, and they influence each other and play a synergistic role in regulation. This study paved the way for further revealing the response mechanism of rice to flooding stress, and demonstrated the importance of the ACC synthase gene family in rice stress resistance, providing a theoretical basis for the cultivation of new waterlogging resistant rice.

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