目录
摘要Ⅱ
关键词Ⅱ
AbstractⅢ
引言
1背景介绍1
1.1拟南芥耐冷胁迫机制1
1.2 6mA研究现状2
1.3 6mA修饰与拟南芥冷胁迫之间的关系2
2材料与方法3
2.1数据来源3
2.2 6mAIPseq数据分析3
2.3 6mA修饰水平变化基因的GO富集4
3 结果与分析4
3.1 样本信息与数据质量控制4
3.2 6mA修饰水平在拟南芥冷处理前后的变化5
3.3 6mA修饰水平变化基因的GO富集7
3.4 6mA修饰水平变化基因与拟南芥冷胁迫之间的关联 8
3.4.1 6mA修饰水平上升的基因与拟南芥冷胁迫之间的关系 8
3.4.2 6mA修饰水平未明显变化的基因与拟南芥冷胁迫之间的关系 9
4 讨论与展望 10
致谢11
参考文献12
拟南芥冷处理前后N6甲基腺嘌呤修饰的全基因组改变及功能分析
摘 要
N6甲基腺嘌呤(6mA)目前被普遍认为是生物基因组中一种新的表观遗传修饰，在原核生物以及真核生物中都有重要的生物学功能，但是6mA在植物抗冷胁迫中的功能尚不清楚。本文以冷处理前后的拟南芥为研究对象，利用6mA特异抗体进行免疫共沉淀结合高通量测序数据分析(6mAIPseq)发现拟南芥基因组上的6mA在经历冷胁迫处理后整体上呈上升趋势。通过对含有6mA峰的基因进行GO分析，我们发现在冷处理后6mA修饰水平上升的基因与拟南芥应对冷胁迫的相关性较大，而6mA修饰水平未明显变化以及水平下降的基因与拟南芥应对冷胁迫的关联则较小。进一步从中挑选具体的基因进行分析，结果发现6mA水平上升的基因往往上调表达以帮助拟南芥抵御寒冷。这些结果说明6mA这一新型表观遗传修饰在拟南芥抵御寒冷时，可能起到一定的调控作用。
CHANGES OF N6METHYLADENINE UNDER COLD TREATMENT IN ARABIDOPSIS AND ANALYSIS OF RELATED GENES *51今日免费论文网|www.jxszl.com +Q: ￥351916072￥ 

ABSTRACT
N6methyladenine (6mA) is currently widely regarded as a new epigenetic modification in the genome of organisms, which has important biological functions in prokaryotes and eukaryotes. while the function of 6mA in cold resistance of plants is still unclear. In this study, Arabidopsis under cold treatment is used as the research object. Using immunoprecipitation with specific 6mA antibody combined with highthroughtput sequencing technology(6mAIPseq) method, we found that the 6mA on the Arabidopsis genome increases after cold stress. Through GO analysis of genes with 6mA peak, we found that the genes with increased 6mA modification level after cold treatment had a greater correlation with Arabidopsis’ response to cold stress, while the genes with no significant change or decreased in 6mA modification level had a smaller correlation. Selecting related genes to analyse, we discover that genes with increased 6mA levels are often upregulated in expression to help Arabidopsis withstand cold. These results indicate that the new epigenetic modification of 6mA may play a role in regulating Arabidopsis’ resistance against cold.
Key words: 6mA; cold stress; epigenetics; gene regulation
1 背景介绍
1.1 拟南芥耐冷胁迫机制
植物生存位置是固定的，因此对外界环境快速准确的响应对植物完成正常的生长发育有重要的作用。随着环境因素的变化，植物也会改变自身的状态以适应环境。温度是植物生长环境的重要因素之一，低温是严重影响植物生长发育以及产量的非生物胁迫。

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