目录
摘要Ⅰ
关键词Ⅰ
AbstractⅡ
引言
引言1
1材料与方法2
1.1试验材料与设计2
1.2测定内容与方法 2
1.2.1主茎叶叶水势测定2
1.2.2棉铃对位叶光合作用和叶绿素荧光测定2
1.2.3棉铃对位叶碳代谢相关物质含量、相关酶活性测定2
1.3数据统计与分析 4
2结果与分析4
2.1干旱对棉花主茎叶凌晨叶水势的影响4
2.1干旱对棉铃对位叶光合作用的影响4
2.3干旱对棉铃对位叶荧光特性的影响 6
2.4干旱对棉铃对位叶碳水化合物含量的影响 7
2.4.1可溶性糖总含量7
2.4.2蔗糖含量8
2.4.3淀粉含量8
2.5干旱对棉铃对位叶碳代谢相关酶活性的影响 9
2.5.1 SPS活性9
2.5.2 SuSy活性9
3讨论与结论 10
3.1花铃期干旱对不同棉花品种叶片光合作用的影响10
3.2花铃期干旱对不同棉花品种叶片荧光特性的影响 11
3.3花铃期干旱对不同棉花品种叶片碳代谢的影响11
3.4结论12
致谢12
参考文献13不同基因型棉花叶片响应干旱胁迫的生理机制研究
摘要
棉花是一种具有重要经济价值的纤维作物，其棉铃的生长发育和纤维的形成与棉铃对位叶的光合作用和碳代谢密不可分。目前，干旱因其时空广泛性而严重影响棉花生产，这在花铃期发生干旱胁迫时表现得尤其明显。同时，在抗旱基因型不同的棉花品种之间，叶片的光合作用和碳代谢对干旱胁迫的响应亦存在差异。本研究选择耐旱型棉花品种德夏棉1号和干旱敏感型棉花品种豫早棉9110为材料，于2019年进行花铃期干旱盆栽试验，设置对照（SRWC(75±5)%）和干旱（SRWC(45±5)%）两个水分处理，探究棉铃对位叶的光合作用和碳代谢响应花铃期干旱胁迫的生理机制并揭示其品种间差异。结果表明：花铃期干旱38天导致两品种棉铃对位叶的净光合速率（P *51今日免费论文网|www.jxszl.com +Q: ^351916072* 
n）、气孔导度（Gs）、胞间CO2浓度（Ci）、最大光化学量子产量（Fv/Fm）和实际光化学量子产量（ΦPSⅡ）均显著降低，非光化学淬灭（NPQ）则表现出上升趋势，各参数在干旱处理间的变化幅度品种间差异不显著，说明花铃期干旱胁迫会明显降低棉铃对位叶的光合生产能力，但是对不同品种的影响差异性较小；花铃期干旱38天导致两品种棉铃对位叶的可溶性糖、蔗糖含量和磷酸蔗糖合成酶（SPS）、蔗糖合成酶（SuSy）的活性均显著增加，淀粉含量则显著降低，其中，德夏棉1号的变化幅度均小于豫早棉9110。因此，花铃期干旱显著影响棉铃对位叶光合作用和碳代谢，导致可溶性糖积累在棉铃对位叶中，从而影响了对棉铃的营养供给，干旱对抗旱品种的影响相对更小；对位叶碳代谢的响应差异可能是导致不同干旱敏感型棉花品种抗旱能力差异的原因之一。
STUDY ON PHYSIOLOGICAL MECHANISM OF DIFFERENT GENOTYPE COTTON LEAVES IN RESPONSE TO DROUGHT STRESS
ABSTRACT
Cotton is a very important fiber crop. Drought stress can significantly affect cotton growth, leaf photosynthesis, carbon metabolism and fiber formation during the flowering and bollforming period, however, the response varied among cotton varieties with different drought resistance. To study the photosynthesis and carbon metabolism differences of subtending leaves between different drought resistance varieties in response to drought stress, Dexiamian 1 (drought tolerant) and Yuzaomian 9110 (drought sensitive) were selected as materials and pot experiment with two water treatments during the flowering and bollforming period was set in 2019: control (SRWC (75 ± 5 )%) and drought (SRWC (45 ± 5)%). Results showed that net photosynthetic rate (Pn), stomatal conductance (Gs), intercellular CO2 concentration (Ci), maximum photochemical quantum yield (Fv / Fm) and actual photochemical quantum yield (ΦPSⅡ) of leaves subtending to cotton bolls were significantly reduced, the nonphotochemical quenching (NPQ) was significantly increased under drought condition. The variation range of each index between water treatments was not significantly differed among varieties. Besides, soluble sugar and sucrose content, activity of phosphate sucrose synthase (SPS) and sucrose synthase (SuSy) were significantly increased, while starch content was significantly reduced by drought. The change range of Dexiamian 1 was smaller than that of Yuzaomian 9110. Therefore, drought during the flowering and bollforming period significantly affected the carbon metabolism of subtending leaves, resulting in the accumulation of soluble sugar in the subtending leaves, which affected the carbohydrate supply to the cotton bolls. Droughtresistant variety was relatively less affected. The difference between responses of leaf carbon metabolism to drought might be one of the reasons in possessing varied droughtresistant ability among varieties.

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