目录
摘要Ⅱ
关键词Ⅱ
AbstractⅢ
引言
引言1
1材料与方法2
1.1样品采集与处理 2
1.1.1土培实验 2
1.1.2土壤铵态氮和硝态氮含量的测定2
1.1.3 土壤DNA提取及保存2
1.1.4土壤中ARGs的定性检测3
1.1.5土壤中ARGs的定量检测4
2结果与分析5
2.1土壤铵态氮和硝态氮含量分析5
2.2土壤中ARGs的定性检测分析 6
2.3土壤中ARGs的定量检测分析7
3讨论11
致谢11
参考文献12
施用不同氮素对土壤中抗生素抗性基因丰度的影响
摘 要
本研究旨在了解使用不同氮素对土壤中抗生素抗性基因丰度的影响，其中氮素使用硝态氮、铵态氮水溶性形式添加，其中硝态氮由NaNO3提供，铵态氮由(NH4)Cl提供，研究氮素添加量分别为0、50、100、200mg/kg时的土壤中的抗生素抗性基因丰度。由各抗性基因的相对丰度图可得知，随着培养时间，各抗性基因丰度均呈现先下降在上升的趋势。这可能是由于培养过程中在光照培养箱的密闭以及外加水分，使的抗性基因丰度下降，后又逐渐恢复。加氮素会使抗性基因丰度先下降后上升的趋势更加明显。随着外加氮素浓度，各抗性基因基本呈现先升高后降低的趋势，外加100mg/kg氮素容易获得抗性基因相对丰度的最大值。这就说明外加氮素会增加抗性基因的丰度，但过量施用会抑制土壤微生物活性，从而使抗性基因丰度降低。不同种类的抗性基因在土壤中的丰度差异一至二个数量级。抗性基因种类不同，氮素对其影响也不同。随着外加氮素浓度，各抗性基因基本呈现先升高后降低的趋势，外加100mg/kg氮素容易获得抗性基因相对丰度的最大值。这就说明外加氮素会增加抗性基因的丰度，但过量施用会抑制土壤微生物活性，从而使抗性基因丰度降低。
EFFECT OF DIFFERENT NITROGEN APPLICATION ON GENE ABUNDANCE OF ANTIBIOTIC RESISTANCE IN SOIL *51今日免费论文网|www.jxszl.com +Q: @351916072@ 

ABSTRACT
The purpose of this study is to understand the effect of different nitrogen application on the antibiotic resistance gene abundance in soil, in which nitrogen is added in the watersoluble form of nitrate nitrogen and ammonium nitrogen, in which nitrate nitrogen is provided by NaNO3 and ammonium nitrogen is provided by (NH4) Cl, and to study the antibiotic resistance gene abundance in soil with nitrogen addition of 0, 50, 100 and 200mg / kg respectively. According to the relative abundance map of each resistant gene, the abundance of each resistant gene first decreased and then increased with the culture time. This may be due to the airtightness of the light incubator and the addition of water during the cultivation process, which makes the resistance gene abundance decrease and then gradually recover. The trend of resistance gene abundance first decreased and then increased was more obvious when adding nitrogen. With the concentration of nitrogen, the resistance genes increased first and then decreased. The maximum relative abundance of resistance genes was easily obtained by adding 100mg / kg nitrogen. This indicated that the addition of nitrogen would increase the abundance of resistance genes, but excessive application would inhibit the activity of soil microorganisms, thus reducing the abundance of resistance genes. The abundance of different resistance genes in soil varied by one or two orders of magnitude. The effects of nitrogen on resistance genes were different. With the concentration of nitrogen, the resistance genes increased first and then decreased. The maximum relative abundance of resistance genes was easily obtained by adding 100mg / kg nitrogen. This indicated that the addition of nitrogen would increase the abundance of resistance genes, but excessive application would inhibit the activity of soil microorganisms, thus reducing the abundance of resistance genes.

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