目录
摘要
关键词
Abstract
引言
引言
1.1研究意义.....................................................................................................2
1.2 植物次生细胞壁的研究进展....................................................................2
1.2.1 植物细胞壁.........................................................................................2
1.2.2 棉花纤维发育.....................................................................................3
1.3 NAC转录因子调控植物次生壁合成的研究进展....................................4
1.3.1 NAC转录因子的结构特征 ...............................................................4
1.3.2 NAC转录因子在拟南芥次生壁合成中的调控作用.........................4
1.3.3 NAC转录因子在杨树次生壁合成中的调控作用.............................5
1.3.4 NAC转录因子在水稻和玉米次生壁合成中的调控作用.................5
1.3.5 棉花NAC转录因子研究进展...........................................................6
二、实验材料................................................................. *景先生毕设|www.jxszl.com +Q: *351916072* 
......................................6
2.1植物材料.....................................................................................................6
2.2菌种和载体.................................................................................................6
2.3化学试剂.....................................................................................................7
2.4工具酶.........................................................................................................7
2.5常用储存液.................................................................................................7
2.6常用缓冲液.................................................................................................7
2.7培养基.........................................................................................................7
2.8 PCR分析扩增试剂.....................................................................................7
2.9 仪器设备.....................................................................................................7
三 实验方法..........................................................................................................8
3.1拟南芥的栽培..............................................................................................8
3.2农杆菌介导的拟南芥的遗传转化..............................................................8
3.3大肠杆菌感受态的制备..............................................................................9
3.4碱裂解法提取质粒......................................................................................9

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