根据肝胆湿热中医临床表现，探讨建立符合中医肝胆湿热证的动物模型。采用 “外湿+高脂高糖+白酒+α-异硫氰酸萘酯（ANIT）”、“高脂高糖+白酒+ANIT”、“外湿+高脂高糖+ANIT”、“普通饲料+ANIT”、“外湿+高脂高糖+白酒” 诱导动物肝内胆汁淤积建立肝胆湿热模型，分别观察不同模型对于小鼠活动状态、生长、肝脏功能、血清胆红素、肝脾指数、肝脏形态的影响。结果表明 与其他四组及空白组相比，“外湿+高脂高糖+白酒+ ANIT”组肝内胆汁淤积、小鼠胆汁排泄量、明显减少，血清TBIL和血清AST、ALT、ALP值均明显增高，同时肝细胞坏死、脾肿大明显。综上“外湿+高脂高糖+白酒+ ANIT”模型与中医肝胆湿热证相似，适合作为肝胆湿热模型。
目录
摘要Ⅱ
关键词Ⅱ
AbstractⅡ
引言
引言
1 材料与方法 1
1．1 实验动物 1
1．2 实验试剂 1
1．3 实验仪器 1
1．4实验方法 1
1．4．1 动物分组、模型制备 1
1．4．2小鼠体征观察 1
1．4．3肝功能检测 1
1．4．4统计方法 1
2 结果 1
2．1 不同模型对小鼠日常活动状态的影响 1
2．2 不同模型对小鼠生长的影响 2
2．3 不同模型对小鼠肝功能的影响 4
2．4 肝指数、脾指数 4
2．5不同模型对肝脏组织形态的影响 5
3 讨论 5
3．1 肝胆湿热造模方法 6
3．2 肝胆湿热动物模型展望 6
致谢 8
参考文献 9
肝胆湿热造模方法的研究
中药学 杜悦
Study on the method of establishing the model of dampheat in liver and gallbladder
traditional Chinese pharmacology DuYue
Tutor Shi Ho *51今日免费论文网|www.51jrft.com +Q: &351916072& 
ngzhuan
Abstract：According to the clinical manifestation of dampheat Syndrome of liver and gallbladder, the animal model of dampheat Syndrome of liver and gallbladder was established. The model of hepatobiliary dampness and heat was established by using "external dampness + high fat and high sugar + liquor +ANIT " , "external dampness + high fat and high sugar + liquor + ANIT" , "common feed + ANIT" , "external dampness + high fat and high sugar + Liquor" , the effects of different models on activity state, growth, liver function, serum Bilirubin, liver and Spleen Index and liver morphology were observed. The results showed that compared with the other four groups and the blank group, the intrahepatic cholestasis, bile excretion and ALP of mice in the "external dampness + high fat + high sugar + white wine + ANIT" group were significantly reduced, and the serum Tbil, AST, ALT and ALP were significantly increased, at the same time, hepatocyte necrosis and splenomegaly were obvious. To sum up, the model of "external dampness + high fat + High Sugar + liquor + ANIT" is similar to the syndrome of dampheat in liver and gallbladder of TCM, so it is suitable to be used as the model of dampheat in liver and gallbladder.
Key words: Dampheat of liver and gallbladder；Animal Model；Symptoms of traditional Chinese medicine；Molding method
中医证候动物模型建立在中医整体观和辨证论治思想基础上，运用脏腑理论和中医病因病机学说理论，在动物身上模拟和复制人体疾病原型的某些特征，使人体疾病症状和病理变化与证型相同或相似的动物 [1]。中医结合动物模型是中医基础方向研究和临床应用之间的桥梁和纽带，同时也是深化中医基础和中医药作用机制研究的手段和方法[2]。
肝胆湿热证是湿热证中最常见症候之一，中医肝胆湿热证表现为湿热内蕴、肝胆疏泄失常等证候，为中医临床常见证候之一。其病因病机多为湿热毒邪外侵，侵犯肝胆经，或饮食肥甘，酿生湿热，或脾胃纳运失常，导致湿浊内生，郁结化热，湿热蕴滞于肝胆。临床研究发现肝胆湿热证与肝内胆汁淤积密切相关，患者肝脏发生显著的肝细胞内和胆管内胆汁淤结等病理学改变以及血清ALT、AST、TBIL等增高[36]。因此常通过探索胆汁淤积的形成机制来研究肝胆湿热证。许多具有清利湿热归肝经的药均具有清肝胆湿热的作用，常涉及到现代多种肝胆疾病[7]，如病毒性肝炎、胆囊炎、淤胆性肝炎、脂肪性肝病、胆结石、肝癌等。由于缺乏一种理想的肝胆湿热动物模型，对肝胆湿热药物作用机制的研究变得十分困难。 因此，理想的中医证候动物模型的建立对于疾病的治疗和中医药的发展具有重要意义。

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