目录
摘要....................................................................................................1
关键词....................................................................................1
Abstract........................................................................................1
Key words....................................................................................................1
引言...............................................................................................................1
1.材料与方法...........................................................................................3
1.1植物材料处理和仪器试剂.........................................................................3
1.1.1植物材料..................................................................................................3
1.1.2仪器与试剂.............................................................................................3
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.2总RNA提取与cDNA合成..................................................................3
1.3 MYBF基因的克隆..........................................................3
1.4序列分析比与进化树构建...........................................................4
1.5理化性质分析和跨膜区域分析......................................................4
1.6 MYBF基因表达特性分析................................................................................4
2.结果与分析..................................................................................4
2.1 MYBF基因的生物信息学分析.............................................................................5
2.1.1 MYBF基因全长cDNA的克隆........................................................5
2.1.2理化性质分析和跨膜区域分析............................................................6
2.1.3 MYB基因的进化树构建...................................................................................6
2.2 MYBF基因表达特性分析................................................................................7
3.讨论...............................................................................................8
致谢..................................................................................9
参考文献..............................................................................9
杭菊中调控黄酮醇合成的MYBF基因的克隆与表达特性分析
学生 张玮婷

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