目录
摘要II
关键词II
AbstractII
引言
引言1
1 材料与方法1
1.1 材料 1
1.1.1 供试材料1
1.1.2 主要试剂1
1.1.3 主要仪器设备1
1.2 试验设计 2
1.2.1 盆栽实验2
1.2.2 实验指标测量2
1.3 实验指标的测定方法 2
1.3.1 叶片中超氧化物歧化酶（SOD）活性的测定2
1.3.2 叶片中过氧化物酶（POD）活性的测定3
1.3.3 叶片中脯氨酸含量的测定3
1.3.4 土壤多糖含量的测定4
1.3.5 土壤渗水率的测定4
1.3.6 统计分析4
2 结果与分析4
2.1 腐殖酸和植物促生菌对黑麦草生长状况的影响4
2.2 腐殖酸和植物促生菌对黑麦草抗逆性的影响6
2.2.1 腐殖酸和植物促生菌对黑麦草叶片中SOD活性的影响6
2.2.2 腐殖酸和植物促生菌对黑麦草叶片中POD活性的影响6
2.2.3 腐殖酸和植物促生菌对黑麦草叶片中脯氨酸含量的影响7
2.3 腐殖酸和植物促生菌对砒砂岩土壤的影响7
2.3.1 腐殖酸和植物促生菌对土壤持水能力的影响7
2.3.2 腐殖酸和植物促生菌对土壤多糖含量的影响8
3 讨论 9
3.1 黑麦草抗逆性指标的变化9
3.2 砒砂岩土壤指标的变化10
致谢11
参考文献11
腐殖酸和植物促生芽孢杆菌联合强化黑麦草修复改良砒砂岩的研究
摘 要
本研究运用腐殖酸和植物促生芽孢杆菌联合强化黑麦草改良修复砒砂岩的方法，试图改良黑麦草在砒砂岩上生长情况，修复砒砂岩水土流失问题，为微生物 强化植物改良砒砂岩土壤的科学设想提供新思路。以黑麦草和砒砂岩为实验材料，设置10个处理组进行盆栽实验，通过测定黑麦草 *51今日免费论文网|www.51jrft.com +Q: ￥351916072$ 
叶片中的SOD活性、POD 活性、脯氨酸含量等植物抗逆性指标和土壤多糖含量、土壤中细菌总数、土壤渗水率等土壤指标探究腐殖酸和植物促生芽孢杆菌联合强化黑麦草修复砒砂岩的效果和机理。结果表明 ：与空白对照组相比，植物促生菌Bacillus spp. P75或HB25发酵液与腐殖酸协同作用的处理组分别显著提高黑麦草叶片中SOD活性171 .0%、251.5%，POD活性83.6%、107.9%，脯氨酸含量129.8%、116.2%等抗逆性指标；显著增加土壤多糖含量171.7%、299.3%，土壤持水能力明显提高。植物促生菌P75或HB25发酵液与腐殖酸肥料协同作用能够显著地提高黑麦草的抗逆性，强化植物对砒砂岩的改良修复。
EFFECTS OF HUMIC ACID AND PLANT GROWTH PROMOTING BACILLUS SPP. ON THE REMEDIATION OF PISHA SANDSTONE SOIL BY PERENNIAL RYEGRASS
ABSTRACT
In order to improve the growth of plants on the pisha sandstone and repair the soil erosion of the pisha sandstone, a new idea was provided for the scientific assumption of pisha sandstone soil improvement by microbial and plants. In this study, humic acid and plantgrowth promoting bacillus were used to strengthen the remediation of pisha sandstone soil by perennial ryegrass. 10 treatments group of potted plant experiment were setting up to explore the effect and mechanism of the combination of humic acid and plant growth promoting Bacillus spp. P75 or HB25 to strengthen the remediation of pisha sandstone soil by perennial ryegrass, through the determination of perennial ryegrass leaf SOD activity, POD activity and proline content in plant resistance index and soil polysaccharide content, the total number of bacteria in the soil, soil water penetration rate index. The results showed that: compared with the control group, the treatment group with the synergic effect of strains P75 or HB25 fermentation broth and humic acid significantly increased SOD activity 171.0%, 251.51%, respectively, POD activity 83.60%, 107.93%, proline content 129.76%, 116.18% and other stress resistance indexes in perennial ryegrass leaves. The soil polysaccharide content was significantly increased by 171.66% and 299.33%, and the soil water holding capacity was significantly improved. The combined action of fermentation broth of plant growth promoting bacteria P75 or HB25 and humic acid fertilizer can significantly improve the stress resistance of perennial ryegrass and strengthen the remediation of plants to pisha sandstone.

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