目录
摘要Ⅱ
关键词Ⅱ
AbstractⅡ
引言
引言（或绪论）1
1材料与方法1
1.1实验材料 1
1.1.1其他材料 1
1.1.2筛选遗传相关材料 2
1.2实验方法 2
1.2.1拟南芥pi4kβ1 pi4kβ2的表型分析 2
1.2.2 PI4Kβ/PtdIns4P在微管上的分布 2
1.2.3BiFC或分离荧光素酶（Luciferase,LUC）验证互作2
1.3数据处理2
2结果与分析3
2.1.1拟南芥pi4kβ1 pi4kβ2的表型分析3
2.1.2 PI4Kβ/PtdIns4P在微管上的分布4
2.1.3 PI4Kβ1与CLASP的互作4
2.1.3.1分离荧光素酶（Luciferase,LUC）验证互作5
3讨论 5
致谢6
参考文献6
植物磷脂酰肌醇4磷酸（Ptdlns4P）参与调控油菜素内酯（BR）介导的植物生长发育
摘要
油菜素甾醇（Brassinosteroid，BR）是20世纪70年代首次从油菜花粉中分离鉴定出的一种甾醇类化合物，其广泛存在于植物体内，对植物的生长发育有重要影响，能有效缓解多种不良因素对植物造成的伤害，增强植株抗性。目前，BR信号转导通路已基本阐明，但其在介导植物生长发育的过程中如何被调控还有待进一步研究。植物磷脂酰肌醇4磷酸（Ptdlns4P）是参调控BR介导的植物生长发育过程中的重要因子。本论文将阐明PI4Kβ/PtdIns4P是如何参与调控油菜素内酯介导的植物生长发育。本研究从生理生化、细胞和遗传的角度发现PI4Kβ/PtdIns4与微管蛋白参与BR信号。PI4Kβ1与微管结合蛋白CLASP互作，稳定调控BRI1的丰度，影响BR反应。旨在于解析拟南芥PI4Kβ/PtdIns4P响应油菜素内酯的分子机理，进一步完善磷脂介导的囊泡运输调控油菜素内酯反应的网络，为今后农作物遗传改良提供切实可靠的理论基础。
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INOSITOL4PHOSPHATE (PTDINS4P) REGULATES BR TO MEDIATED PLANT GROWTH AND DEVELOPMENT
ABSTRACT
Brassinosteroid is a sterol compound isolated and identified from rape pollen for the first time in the 1970s. It is widely present in plants and has an important impact on the growth and development of plants. It can effectively alleviate the damage caused by various adverse factors to plants and enhance it resistance. At present, the BR signal transduction pathway has been basically elucidated, but how it is regulated in the process of mediating plant growth and development remains to be further studied. Ptdlns4P is an important factor involved in regulating BRmediated plant growth and development. We will clarify PtdIns4P regulates BR to mediated plant growth and development. And via physiology, biochemistry, cell and genetics, we found that the interaction between PI4Kβ/PtdIns4 and tubulin depends on the involvement of microtubules in BR signaling. And we also found PI4Kβ1 regulate the richness of BRI1 stably and influence the reaction of BR, which is dependent on microtubule binding protein, CLASP. We aimed to analyze the molecular mechanism of Arabidopsis PI4Kβ/PtdIns4 response to BR, and further improve the network of phospholipidmediated vesicle transport to regulate BR reaction, so as to provide a reliable theoretical basis for future crop genetic improvement.
KEY WORDS: BR ; Ptdlns4P ; microtubule ; PI4Kβ1 ; PI4Kβ2
植物生长发育过程中的调控机制一直是植物研究的重点。而囊泡运输广泛参与调控植物的生长发育、细胞生长、分裂和抗逆应答等重要过程。囊泡运输能将脂类、蛋白质和一些其他物质运输到特定的膜上，从而进行细胞的传递，使得细胞对外界和自身的环境变化做出响应。

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