目录
摘要II
关键词II
AbstractIII
引言
引言（或绪论）1
1□材料与方法2
1.1□实验材料 2
1.2□实验方法 2
1.2.1□土壤样品采集2
1.2.2□土壤微生物多样性测定2
2□结果与分析3
2.1□土壤微生物组学测序数据分析3
2.2 微生物多样性和群落结构分析4
2.2.1 细菌群落组成情况4
2.2.2 Alpha多样性分析6
2.2.3 Beta多样性分析6
2.2.4 差异统计7
3□讨论 10
3.1轮作对土壤微生物群落的影响10
3.2土壤微生物群落与草莓生长及病害防治的关系10
3.3土壤微生物绝对定量的研究11
致谢12
参考文献12
附录 大棚草莓不同时期长势情况14
根瘤菌—绿豆与草莓轮作中对土壤微生物群落和草莓生长的影响
摘 要
基于根瘤菌—绿豆与草莓轮作的种植模式对草莓长势的促进有显著作用，本文旨在探究轮作后土壤微生物群落的变化及其对草莓生长的作用。以不同时期的草莓根际土壤为样本，16S rRNA基因的高通量测序表明细菌群落组成和多样性发生变化，与对照相比，处理组的细菌多样性下降。主成分分析（PCA）与非度量多维标度（NMDS）分析共同显示了轮作造成的土壤菌群差异，表明种植绿豆后会改变土壤细菌群落的演变速率。线性判别分析（LEfSe）结果表明，变形菌门（Proteobacteria）、放线菌门（Actinobacteria）、绿弯菌门（Chloroflexi）、酸杆菌门（Acidobacteria）和异常球菌—栖热菌门（DeinococcusThermus）是具有潜在功能的重要差异土壤微生物菌群。与播种绿豆前初始期（CG0）土样相比，轮作绿豆后的草莓成熟结果期（GSF）的根际土壤中固氮细菌和光合细菌等有益菌群类型有明显优化，在草莓的生长及病害防治过程中可能存在一定的作用。更系统地还需结合土壤真菌群落的ITS序列和RTqPCR *51今日免费论文网|www.51jrft.com +Q: *351916072* 
（Realtime quantitative Polymerase Chain Reaction）进一步分析真菌的种类和数量变化，为构建良好的根际微生物区系和健康的草莓土壤生态系统提供参考。
EFFECTS OF RHIZOBIUMMUNG BEAN ON SOIL MICROBIAL COMMUNITY AND STRAWBERRY GROWTH IN STRAWBERRY ROTATION
ABSTRACT
Based on the result that rotation of rhizobiummung bean and strawberry had a significant effect on promoting the growth of strawberry, the objective of this study was to investigate the influence of rotation on soil microbial community and strawberry growth. The rhizosphere soil samples of strawberry in different periods were taken as samples. Highthroughput sequencing of 16S rRNA genes demonstrated that the bacterial community composition and diversity changed. Compared with the control group, the bacterial diversity of the treatment groups decreased. Principal component analysis (PCA), in combination with nonmetric multidimensional scaling (NMDS) analysis, showed differences of bacterial community composition in the rotation system, indicating that planting mung beans changed the evolutionary rate of soil bacterial community. Results of linear discriminant analysis effect size (LEfSe) revealed that Proteobacteria, Actinobacteria, Chloroflexi, Acidobacteria and DeinococcusThermus were the important variant microbial groups, potentially functional in soil. The rhizosphere soil in strawberry at the fruit stage (GSF) contained more effective micoorganisms, such as nitrogenfixing bacteria and photosynthetic bacteria, compared with the initial soil samples before sowing mung beans (CG0), which may play a role in the growth and disease control of strawberry. More systematically, it is necessary to combine the ITS sequence of soil fungal community and Realtime quantitative Polymerase Chain Reaction to further analyze the species and quantities of fungi, which will provide reference for the construction of good rhizosphere microbial flora and healthy strawberry soil ecosystem.

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