目录
摘要II
关键词II
AbstractIII
引言
引言1
1材料与方法2
1.1数据库 2
1.2大豆KNOX基因的查找及大豆KNAT7基因的查找 2
1.2.1大豆KNOX基因的查找2
1.2.2大豆KNAT7基因的查找2
1.3大豆KNOX蛋白系统发育树分析2
1.4大豆KNOX基因表达分析3
1.5大豆KNAT7蛋白的系统发育分析3
1.6大豆KNAT7基因表达分析3
1.7大豆KNAT7蛋白多序列比对 3
1.8大豆KNAT7基因结构分析3
1.9大豆KNAT7基因的比较基因组分析3
2结果与分析4
2.1大豆KNOX基因及KNAT7基因的查找结果4
2.2大豆KNOX蛋白的系统发育分析结果5
2.3大豆KNOX基因表达分析结果6
2.4大豆KNAT7蛋白的系统发育分析结果7
2.5大豆KNAT7基因表达分析结果8
2.6大豆KNAT7蛋白多序列比对结果9
2.7大豆KNAT7基因结构分析结果10
2.8大豆KNAT7基因的比较基因组分析结果10
3讨论11
致谢13
参考文献13
大豆GmKNAT7基因的克隆和表达分析
摘 要
KNOX基因家族编码的产物是一类含有同源异型盒结构域的转录调控因子，对植物的生长发育和器官分化都起重要作用。本实验根据最新测序完成的大豆(Glycine max) Zhonghuang 13基因组数据，利用生物信息学手段鉴定大豆KNOX基因家族中的KNAT7基因，并分析其基因结构、蛋白保守结构域及基因表达模式。本实验通过数据检索和系统发育树找到了4个大豆KNAT7基因，其中SoyZH13_01G197400和SoyZH13_11G026300亲缘关系较近，SoyZH13_17G128300和SoyZH13_05G049200亲缘关系较近；基因结构分析显示SoyZH13_0 *51今日免费论文网|www.51jrft.com +Q: ￥351916072￥ 
1G197400的基因结构与SoyZH13_11G026300类似，SoyZH13_17G128300的基因结构和SoyZH13_05G049200类似；基因表达分析显示SoyZH13_05G049200在大豆所有组织均有微弱的表达，而SoyZH13_11G026300在所有部位均有不同程度的表达，在子叶、根、茎、叶芽中表达量低，但在叶、花、果荚和种子中表达量高；SoyZH13_17G128300在除子叶外的其他部位均有不同程度的表达，在种子、根、叶芽和花中表达强于其他部位，SoyZH13_01G197400在茎以外的其他部位均有表达，在茎和种子中微弱表达，但在子叶、叶、果荚中高表达。比较基因组分析结果显示在基因复增后，基因所在的DNA区域也发生变化，这可能会引起该基因表达模式的变化，从而引起基因功能的变化。
Cloning and expression analysis of GmKNAT7 gene from soybean
ABSTRACT
The KNOX family genes encode a class of transcriptional regulators containing homeobox domains, which play an important role in plant growth and organ differentiation. Based on the latest sequencing genome data of soybean (Glycine max), Zhonghuang 13 genome data, we used bioinformatics to search for KNAT7 genes in KNOX gene family from soybean and analyzed their gene structure, protein conserved domain and gene expression pattern. Phylogenetic tree showed that SoyZH13_01G197400 and SoyZH13_17G128300 were closely related with SoyZH13_11G026300 and SoyZH13_05G049200, relatively. Gene structure analysis showed that the gene structure of SoyZH13_01G197400 was similar to rhat of SoyZH13_11G026300, while SoyZH13_17G128300 was similar to that of SoyZH13_05G049200. Gene expression analysis showed that the expression patterns of these four KNAT7 genes were very different. SoyZH13_05G049200 expressed weakly in all plant parts. SoyZH13_11G026300 had different levels of expression in all plant parts, but it expressed weakly in seeds, shoot meristem, cotyledons and stems. SoyZH13_17G128300 expressed in varying degrees except for cotyledons, and expressed higher in seeds, roots, leaf buds, and flowers than in other parts. SoyZH13_01G197400 expressed in other parts than the stem, and highly expressed in leaf buds, leaves, flowers, shoot meristem and pods, and its expression was highest in leaves. The results of comparative genomic analysis showed that the environment in which the gene was located also changed after the gene duplication, which may result in a change in the expression pattern of the gene, thereby leading to a functional diversity.

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