目录
摘要Ⅱ
关键词Ⅱ
AbstractⅢ
引言
引言1
1.材料与方法1
1.1材料1
1.2表达载体的构建及转化2
1.3拟南芥培养2
1.4农杆菌侵染拟南芥2
1.5 转GsCLCb1基因拟南芥的筛选2
1.6转基因拟南芥的培育及盐处理3
1.7测定方法 3
1.7.1Na+、K+含量测定 3
1.7.2Cl含量测定 3
1.7.3NO3 含量测定 3
1.7.4DAB和NBT染色4
2.结果与分析4
2.1盐胁迫下拟南芥种子萌发率的比较4
2.2根伸长量及单株鲜重比较5
2.3盐胁迫下GsCLCb1基因对拟南芥离子含量的影响 6
2.3.1根部离子含量的变化6
2.3.2地上部离子含量的变化7
2.4活性氧（H2O2、O2）的测定8
3.讨论9
致谢10
参考文献11
大豆CLCb1基因的耐氯（盐）性功能分析
摘要
现如今，土壤盐渍化问题严重，大豆是我国主要粮食作物，分析大豆GsCLCb1基因有利于丰富大豆耐氯性功能研究。本研究通过构建含GsCLCb1的表达载体，并使用花序侵染法将重组质粒导入拟南芥野生型（WT）、突变体（atclcb）。对四种拟南芥NaCl处理后，测定四种拟南芥的萌发率、根长、鲜重、离子含量、H2O2和O2含量来分析大豆GsCLCb1基因的耐氯性。研究结果表明：从表观看，野生型和突变体拟南芥受NaCl胁迫影响，生长受抑制，萌发率低，叶片发黄，根短小，鲜重低。突变体受抑制程度更明显，植株发白死亡。两个转基因拟南芥生长状况良好，受抑制程度不明显。从生理状况看，野生型和突变体拟南芥的Cl、H2O2、O2含量上升。对比两个未转基因的拟南芥，转基因拟南芥的地上部Cl含量、H2O2和O2含量下降。这说明大豆GsCLCb1基因转化到野生型和突变体拟南芥中，可以提高耐氯（盐）性。
CHLORIDE (SALT) RE *51今日免费论文网|www.51jrft.com +Q: &351916072& 
SISTANCE FUNCTION ANALYSIS OF SOYBEAN CLCB1 GENE
ABSTRACT
Nowadays, the problem of soil salinization is serious. Soybean is the main food crop in China. Analysis of soybean GsCLCb1 gene is beneficial to enrich the research of soybeans chloride tolerance. In this study, the expression vector containing GsCLCb1 was reconstructed and the recombinant plasmid was introduced into the wildtype Arabidopsis （WT ）and mutant （atclcb）using the inflorescence infection method. After the treatment of Arabidopsis thaliana with NaCl, the measured scientific research results showed that: from the surface, wildtype and mutant Arabidopsis thaliana were affected by NaCl stress, growth was inhibited, germination rate was low, leaves were yellow, roots were short, and fresh weight was low. The mutants were more inhibited and the plants died white. From the physiological point of view, Cl, H2O2, O2content of wildtype and mutant Arabidopsis thaliana was increasing. Contrary to the two nontransgenic Arabidopsis thaliana, Cl content above ground, H2O2, O2content of the transgenic Arabidopsis thaliana was decreasing. This shows that the soybean GsCLCb1 gene transformed into wildtype and mutant Arabidopsis thaliana can improve chloride (salt) resistance.

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