目录
摘要Ⅱ
关键词Ⅱ
AbstractⅢ
引言
引言1
1 材料与方法2
1.1 材料 2
1.1.1 实验菌株2
1.1.2 杜梨幼苗2
1.1.3 实验土壤2
1.2 实验设计 2
1.2.1 设置实验处理2
1.2.2 种植杜梨幼苗3
1.3 植物分析3
1.3.1 测量杜梨株高3
1.3.2 测量杜梨干重3
1.3.3 测量P和K的含量3
1.3.4 测量杜梨根部侵染率和侵染强度3
1.4 土壤酶活性测定3
1.5 数据分析3
2 结果与分析4
2.1 杜梨幼苗株高和生物量4
2.1.1 杜梨幼苗株高4
2.1.2 愈杜梨幼苗生物量4
2.2 矿质元素的吸收5
2.3 杜梨根部侵染率和侵染强度6
2.4 杜梨根际土壤酶活性6
3 讨论和结论 7
3.1 讨论 7
3.2 结论 8
致谢8
参考文献9
丛枝菌根真菌Glomus etunicatum与促生细菌Rhizobium sp. W33对杜梨幼苗生长的影响
摘 要
【目的】研究丛枝菌根真菌Glomus etunicatum（幼套球囊霉，GE）和促生细菌Rhizobium sp. W33及其联合作用对杜梨生长的影响，为研制适合梨树生长的生物菌肥提供试验基础。【方法】本文以杜梨苗为实验材料，利用两种来源的土壤开展盆栽试验，设置4种处理：接灭活菌处理(CK)、接种W33、接种GE和双接种处理W33+GE，研究菌株对杜梨苗生长、矿质元素吸收和土壤酶活性的影响。【结果】GE和W33均可促进杜梨幼苗在两种供试土壤中的株高，以及地上部和地下部干重，P或K的吸收，GE对杜梨生长的促进作用要优于W33。与GE相比，双接种处理并未发现明显的促生作用。接种GE显著提高杜梨根部菌根的侵染率和侵染强度，也明显高于双接种处理（p<0. *51今日免费论文网|www.51jrft.com +Q: *351916072* 
05）。接种W33和GE均可提高土壤蔗糖酶和脲酶活性，GE还会提高中性磷酸酶活性。与GE处理相比，双接种处理未表现出更高的土壤酶活性。【结论】W33和GE均可促进杜梨幼苗的生长，以及对矿质元素的吸收。但是双接种处理并未表现出明显的协同作用，其综合效果要低于GE处理。
EFFECT OF ARBUSCULAR MYCORRHIZAL FUNGAL GLOMUS ETUNICATUM AND PLANT GROWTHPROMOTING RHIZOBIUM SP. W33 ON THE GROWTH OF PYRUS BETULAEFOLIA
ABSTRACT
【Objective】Understanding the effects of arbuscular mycorrhizal fungal Glomus etunicatum and plantgrowthpromoting Rhizobium sp. W33 alone or in combined on the growth and nutrient uptake of Pyrus betulaefolia will provide us essential information to develop new biological fertilizer.【Methods】In this study, pot experiments were carried out in a greenhouse and the related soils were collected from two commercial pear orchards, Baima and Hushu. The treatments were following: inoculation with (1) dead microbe control (CK), (2) W33, (3) GE, (4) W33+GE. The growth parameters including height and root and shoot dry weight were determined, the activities of invertase, urease and neutral phosphatase were also considered.【Results】The data showed that in both tested soils, inoculation with W33 or GE leaded to a significant improvement in height, shoot and root dry weight, and absorption of P and/or K. Inoculation with GE showed better effects on the growth of P. betulaefolia than that with W33 (p < 0.05). Compared with GE, no effect was observed when the seedling were inoculated with W33+GE. GE and W33+GE treatments increased the colonization frequency and intensity, but not for W33, and GE exhibited the highest values of them (p < 0.05). Inoculation with W33 and GE significantly improved the activities of invertase and urease, and GE also played important role in neutral phosphatase (p < 0.05). Comparatively, W33+GE treatment did not show the effect on improving the activities of soil enzymes. 【Conclusion】Microbial strains W33 and GE could significantly promote the growth of P. betulaefolia, showing higher height, contents of P and/or K and activities of soil enzymes. However, coinoculation treatment in both tested soils did not show higher growth parameters, suggesting that synergistic effect between W33 and GE on growth of P. betulaefolia was unreal.

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