目录
摘要Ⅰ
关键词Ⅰ
AbstractⅡ
引言
引言1
1 材料与方法1
1.1 实验材料及仪器 1
1.1.1 植物材料及生长条件 1
1.1.2培养基与营养土 1
1.1.3 实验仪器2
1.2 实验方法2
1.2.1 植物种植2
1.2.2 芯片分析2
2 结果与分析3
2.1 拟南芥sdg8单突变体差异基因分析3
2.1.1 芯片数据分析 3
2.1.2差异基因GO分析 4
2.2 拟南芥sdg7 sdg8双突变体差异基因分析6
2.2.2 芯片数据分析6
2.2.2 差异基因GO分析7
2.3 sdg8单突变体与sdg7 sdg8双突变体差异基因对比9
2.3.1 sdg8单突变体和sdg7 sdg8双突变体中均上调的基因分析10
2.3.2 sdg8单突变体和sdg7 sdg8双突变体中均下调的基因分析12
2.3.3 sdg8单突变体和sdg7 sdg8双突变体中表达差异相反的基因分析13
2.3.4 sdg8单突变体中上调，sdg7 sdg8双突变体中无差异基因分析15
2.3.5 sdg8单突变体中下调，sdg7 sdg8双突变体中无差异基因分析16
3讨论17
致谢18
参考文献18拟南芥组蛋白H3K36me3甲基转移酶在不同生长条件下的转录差异
摘要
组蛋白的甲基化是表观遗传学领域的一个重要研究方向，在基因的转录表达等多个方面起调控作用。其中组蛋白H3K36的甲基化与DNA复制、DNA损伤修复以及基因转录起始等多个过程有关。SDG8是拟南芥中的组蛋白甲基化转移酶，主要调控组蛋白H3K36的二甲基化与三甲基化水平，在植物生长的多个方面起着关键作用。SDG7是一类新发现的与SDG8相似的蛋白，也具有H3K36甲基转移酶功能。本课题利用基因芯片技术，测量了拟南芥sdg7 sdg8双 *51今日免费论文网|www.51jrft.com +Q: ^351916072* 
突变体和野生型的转录组数据，并通过美国国家生物技术信息中心（NCBI）的GEO数据库获取sdg8单突变体的转录组数据。利用生物信息学方法分析SDG7的生物学功能以及与SDG8之间的相互关系。结合基因本体论分析和基因表达对比发现，SDG7可能影响植物生长过程中的多个方面，包括植物免疫，育性，植物株型等。
TRANSCRIPTION DIFFERENCES OF ARABIDOPSIS HISTONE H3K36ME3 METHYLTRANSFERASE UNDER DIFFERENT GROWTH CONDITIONS
ABSTRACT
Histone methylation is an important research topic in the field of epigenetics, which plays a regulatory role in many aspects of biological processes, including trabscription. Among them, the methylation of histone H3K36 is related to multiple processes such as DNA replication, repair, and transcription initiation. SDG8 is a histone methyltransferase in Arabidopsis thaliana that can regulate the level of dimethylation and trimethylation of histone H3K36 and plays a key role in many aspects of plant growth. SDG7 is a newly discovered protein similar to SDG8 in protein structure, which also has the H3K36 methyltransferase function. This study uses RNA microarray technology to analyze the transcriptome of Arabidopsis sdg7 sdg8 double mutant. With the transcriptome data of sdg8 mutant obtained from NCBI, the biological function of SDG7 was indicated through the transcriptional comparison between sdg7 sdg8 and sdg8 with bioinformatics methods. Gene ontology analysis and gene expression analysis showed that SDG7 controls many aspects of plant development and environmental responses, including plant immunity, fertility, plant size.
KEY WORDS: Histone methylation；H3K36me3；RNASeq；SDG8; SDG7

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