目录
摘要II
关键词II
AbstractIII
引言
引言（或绪论）1
1 材料与方法2
1.1 供试材料 2
1.2 试验方法 2
1.2.1 实时荧光定量PCR鉴定2
1.2.2 抗盐性分析4
1.2.3 盐胁迫下GFPVamp721的定位分析4
2 结果与分析4
2.1 超表达Vamp721突变体鉴定4
2.2 盐胁迫对拟南芥主根长的影响4
2.3 盐胁迫下GFPVamp721的定位5
3 讨论6
3.1 盐胁迫对拟南芥主根发育的影响6
3.1 盐胁迫下GFPVamp721的定位7
致谢8
参考文献8
图1 Realtime PCR 鉴定结果4
图2 盐胁迫下拟南芥主根长度的变化5
图3 GEP标记的Vamp721的定位于质膜、内体和液泡膜6
拟南芥Vamp721在抗盐胁迫中的功能研究
摘 要
盐胁迫是影响作物生长发育和产量的一种非生物胁迫因素。盐离子浓度过高会导致土壤渗透势提高，植物根部细胞脱水，细胞失去膨压，生理功能受到严重影响。因此，对植物耐盐机制的探索成为了目前研究的热点。有研究表明，植物在抵抗盐胁迫时，与液泡有关的囊泡运输起到了关键作用。本实验通过对野生型拟南芥及PVamp721::GFPVamp721突变型在0 mM、50 mM、100 mM浓度盐处理下主根发育情况的观察测量和GFPVamp721定位分析，探究RSNARE Vamp721在抗盐胁迫中的功能，揭示其在盐胁迫中的作用机理，为改良植物遗传、改善土壤环境等实际应用问题提供理论依据。结果表明，在盐胁迫下，野生型拟南芥的主根生长受到明显抑制，且随着盐浓度的升高，抑制作用更加显著；而Vamp721超表达突变型拟南芥在低盐浓度下则表现出有一定的适应性，高盐浓度下主根长度变短，受到抑制，但是受抑制程度明显小于野生型拟南芥。GFP荧光法定位亚细胞实验表明在盐胁迫响应过程中Vamp721定位于质膜、内体和液泡膜。总之，Va *51今日免费论文网|www.51jrft.com +Q: *351916072* 
mp721参与了拟南芥的盐胁迫响应机制，能提高拟南芥抵抗盐胁迫的能力。
A STUDY OF THE FUNCTION OF ARABIDOPSIS THALIANA Vamp721 IN RESISTANCE TO SALT STRESS
ABSTRACT
Salt stress is an abiotic stress factors that affects crop growth, development and yield.High salt concentration leads to the increase of soil osmotic potential, the dehydration of plant root cells and the loss of cell turgor.Eventually,its physiological function is seriously affected.Therefore, the research on the mechanism of salt tolerance has become a hot topic.Studies have shown that vesicle transport related to vacuoles plays a key role in the process of resisting salt stress.In Arabidopsis thaliana,VAMP721 which belongs to the RSNARE involved in salt stress response mechanism by mediating vesicle fusion process.This experiment observed and tested the growth of the taproot of wildtype Arabidopsis thaliana and PVamp721::GFPVamp721 mutant under salt treatment with concentrations of 0 mM, 50 mM and 100 mM and analysed GFPVamp721 positioning,so as to explore the function of RSNARE Vamp721 in resistance to salt stress, and reveal its mechanism in salt stress, which provides the theory basis for plant genetic improvement,soil environment improvement and other actual application problem.The results showed that the growth of the taproot of wildtype Arabidopsis thaliana was significantly inhibited under salt stress,and with the increase of salt concentration, the inhibition was more obvious. Instead,the Vamp721 overexpressed mutant had adaptability under low salt concentration,while the length of taproot became short and the growth was inhibited under high salt concentration. However, the degree of inhibition was significantly less than that of the wild type.GFP fluorescent subcellular assay shows that Vamp721 positioning within the plasma membrane, body and vacuole membrane in response to salt stress.In a word, Vamp721 participates in the salt stress in response to mechanism of Arabidopsis thaliana, and can improve the ability of resistance to salt stress in Arabidopsis thaliana.

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