GmCLC-c1和GmCLC-c2是大豆中的重要基因，可以参与调控两种电压门控通道蛋白，对大豆的生长发育具有重要的调节作用。为探究这两种基因对大豆耐盐性的影响，本实验构建了干扰载体与过表达载体，并在测序成功后将其转入了发根农杆菌中，然后将切根的大豆幼苗浸泡在活化后的发根农杆菌菌液中，成功得到了GmCLC-c1/2干扰或过表达大豆发根组合植株。本实验设置了120mM NaCl处理和作为对照的0mM NaCl处理各五天，并在处理结束后测量了各组植株的株高，叶面积，相对含水量与各植株根，茎，叶的Cl-含量，NO3-含量，Cl-/ NO3-比值。实验结果表明，盐处理下四种材料植株矮小，叶片皱缩，叶片颜色发黄。而过表达GmCLC-c1/2后大豆幼苗NO3-含量显著提高，根部Cl-含量下降，茎、叶部的Cl-含量提高。综上所述，GmCLC-c1和GmCLC-c2可将Cl-富集于根部，减少Cl-向大豆地上部的转运，同时可以增强大豆对NO3-的吸收，这些共同增强了大豆对盐害的抗性。本实验得到的成果可以为今后解决大豆生长发育过程中遇到的盐害问题提供参考依据。
目录
摘要II
关键词II
AbstractIII
引言
引言1
1材料与方法2
1.1材料 2
1.2 PCR反应体系及反应程序 2
1.3发根农杆菌K599转化3
1.4盐胁迫处理3
1.5 表型观察、形态指标和Cl，NO3含量测定 3
2 结果与分析 4
2.1发根鉴定4
2.2 GmCLCc1/2干扰或过表达对盐胁迫下大豆发根组合植株生长表型和形态指 标的影响 4
2.3 GmCLCc1/2干扰或过表达对盐胁迫下大豆发根组合植株Cl，NO3含量的影 响6
3 讨论7
致谢8
参考文献8
GmCLCc1和GmCLCc2对大豆耐盐性的影响及生理机制
EFFECT OF GMCLCC1 AND GMCLCC2 ON SALT TOLERANCE OF SOYBEAN AND ITS PHYSIOLOGIC *51今日免费论文网|www.51jrft.com +Q: ^351916072# 
AL MECHANISM
ABSTRACT: GmCLCc1 and GmCLCc2 are important genes in soybean, which can regulate two voltagegated channel proteins and play an important role in the tolerance of salt stress during the growth and development of soybean. In order to explore the effects of these two genes on salt tolerance of soybean, interference vector and overexpression vector were constructed in this experiment. After sequencing successfully, they were transferred into Agrobacterium rhizogenes, and then the soybean seedlings with cut roots were soaked in the activated Agrobacterium rhizogenes solution, and GmCLCc1/2 interference or overexpression plants were successfully obtained. In this experiment, 120mM NaCl treatment and 0mM NaCl treatment as control were conducted for five days. After treatment, the plant height, leaf area, relative water content, Cl content, NO3 content and Cl/ NO3 ratio of each plant were measured. The results showed that the growth of the whole plant under salt treatment was worse than that of the control, the shape of the leaves was wilting, the area was small, and the color of the leaves was yellow. However, after overexpression of GmCLCc1/2, the NO3 content of soybean seedlings increased significantly, the Cl content of roots decreased, and the Cl content of stems and leaves increased. In conclusion, GmCLCc1 and GmCLCc2 can enrich Cl in the root, reduce the transport of Cl to the shoot , and enhance the absorption of NO3 in soybean, which together enhance the salt tolerance of soybean. The results of this experiment can provide reference for solving the problem of salt damage in the process of soybean growth and development in the future.
KEYWORDS: salt stress; salt tolerance; GmCLCc1; GmCLCc2；Soybean hairy root composite plant

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