目录
摘要Ⅱ
关键词Ⅱ
AbstractⅢ
引言
引言（或绪论）1
1□材料与方法3
1.1□生物信息学分析 3
1.1.1□数据来源3
1.1.2□七个水稻BRs信号转导关键基因家族成员的初步鉴定 3
1.1.3□水稻七个BRs信号转导关键基因的家族成员的多序列比对和系统发育树构建3
1.1.4□BR信号转导关键基及其同源基因启动子内的顺式调控元件分析4
1.2□OsBRIs、OsSERKs、OsBSKs、OsGSKs、OsBZRs、OsILIs各家族成员及籽粒品质形成关键基因的转录数据分析 4
1.2.1□微阵列数据标准化4
1.2.2□数据统计分析4
1.3□候选基因在籽粒发育中作用探究4
1.3.1□候选基因的选择与鉴定4
1.3.2□候选基因与OsNFYB1籽粒发育初期表达差异分析4
2□结果与分析5
2.1□生物信息学分析5
2.1.1□水稻BRs信号转导关键基因家族成员的鉴定5
2.1.2□水稻BRs信号转导关键基因家族系统发育树构建为研究水稻BRs6
2.1.3□水稻BRs信号转导关键基因家族成员组织表达模式分析7
2.1.4□水稻BRs信号转导关键基因家族成员启动子顺式调控元件分析9
2.2□候选基因与OsNFYB1籽粒发育初期共表达差异分析10
3□讨论13
3.1□BR信号转导组分的保守性、多拷贝及其可能的生物学意义14
3.2□OsGSK5在籽粒发育过程中可能的作用14
3.3□BRs调控籽粒发育品质的其他可能途径14
致谢15
参考文献16
水稻油菜素内酯信号转导关键基因的生物信息学分析及其在籽粒发育中的作用初探
摘 要
植物激素稳态在水稻籽粒发育品质形成中具有重要作用，油菜素内酯（Brassinolides）作为植物中唯一的固醇类激素，在物形态建成、细胞衰老、环境适应等方 *51今日免费论文网|www.51jrft.com +Q: ^351916072^ 
面具有重要作用。然而目前对水稻信号转导组分，及其在籽粒发育中的分子机制还不是十分清楚。本研究通过对水稻七个BR信号转导基因进行进行生物信息学分析，系统发育分析结果显示，油菜素内酯信号转导基因在不同物种内是十分保守的，有趣的是BSKs家族和SERKs家族中的一些成员在不同物种间表现出了一定的保守性。在本次实验中共鉴定BR信号转导基因及其同源基因51个，预测一个BR下游效应因子OsILIs家族新成员。并根据组织表达模式、启动子区含有顺式作用元件筛选出21个与籽粒发育相关的候选基因。从候选基因中挑选与水稻籽粒形成关键基因OsNFYB1同样具有胚乳特异性表达模式的基因OsGSK5进行差异分析。结果显示，BR信号可能通过OsGSK5OsNFYB1调控水稻籽粒发育及品质形成。
Bioinformatic Analysis Of Key Genes of Brassinolide Signal Transduction In Rice(Oryza sativa L.) And Its Role In Grain Development
ABSTRACT
Plant hormone homeostasis plays an important role in the development and quality formation of rice grains. BRs(Brassinolides), as the only steroid hormone in plants, plays an important role in morphogenesis, cell senescence and environmental adaptation. However, at present, the signal transduction components of rice and their molecular mechanism in grain development are not very clear. In this study, seven BR signal transduction genes in rice were analyzed by bioinformatics. The results of phylogenetic analysis showed that brassinolide signal transduction genes were very conservative in different species. Interestingly, some members of BSKs family and SERKs family showed some conservatism among different species. In this experiment, 51 BR signal transduction genes and their homologues were identified, and a new member of BR downstream effector OsILIs family was identified. According to the tissue expression pattern and the presence of cisacting elements in the promoter region, 21 candidate genes related to grain development were identified. The gene OsGSK5, which has the same endospermspecific expression pattern as the key gene OsNFYB1 in rice grain formation, was selected from the candidate genes for coexpression analysis. The coexpression of differential genes showed that BRs signal may regulate rice grain development and quality formation through OsGSK5OsNFYB1.

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