目录
摘要Ⅲ
关键词Ⅲ
AbstractⅣ
引言
第一章 引言1
第二章 文献综述 1
1.大豆抗虫性研究进展 2
1.1大豆抗虫机制 2
1.2大豆主要虫害及种类 2
1.3大豆资源的抗虫研究 2
1.4大豆抗斜纹夜蛾研究 3
2.分子标记 4
2.1.分子标记的类型 4
2.2 SSR分子标记的应用 4
2.3分子标记辅助的概况 4
2.4分子标记辅助选择在大豆抗虫中的应用 5
2.5大豆抗斜纹夜蛾的QTL定位 5
3.研究任务、技术路线5
第三章 材料与方法6
1.1供试材料 6
1.2试验设计方案 6
1.2.1室内养虫7
1.2.2抗虫分子标记辅助选择分析7
第四章 结果与分析7
1.F2群体对斜纹夜蛾的抗生性分析7
1.1 斜纹夜蛾幼虫重的频数分布7
1.2 斜纹夜蛾幼虫重抗生性描述7
2.2 抗虫分子标记辅助选择分析8
2.2.1 F2群体的单标记分析8
2.2.2 F2群体的双标记分析9
第五章 结论与讨论10
5.1 结论与分析10
5.2讨论与展望10
致谢10
参考文献11
大豆对斜纹夜蛾抗虫性的分子标记辅助选择研究
摘要
斜纹夜蛾是一种对大豆产量和品质影响很大的食叶性害虫。化学杀虫剂仍是现在主要的斜纹夜蛾防治手段，然而化学防治污染重、危害大影响食品安全并且传统抗虫育种也有很多困难，因此有效防治虫害，减少杀虫剂过量使用是大豆防治虫害的趋势。本研究采取室内喂养斜纹夜蛾的方法，使用田间采集的大豆资源的叶片，在室内喂养斜纹夜蛾的幼虫，以6、9、12天的斜纹夜蛾幼虫重为指标对斜纹夜蛾单一虫种的大豆抗性表现进行鉴定。以第12天的斜纹夜蛾幼虫重的方差分析及其多重比较对大豆组合CSSL59×RIL227的F2 *51今日免费论文网|www.51jrft.com +Q: ^351916072* 
代群体进行抗斜纹夜蛾的分子标记分析，位点Sat_258及Satt626的F值都达显著水平，表明幼虫重差异显著。Sat_258位点含有CSSL59等位变异的植株数为24个，抗虫等位变异较感虫等位变异幼虫重量显著降低了14.7%。Satt626位点含有CSSL59、LY227和杂合等位变异植株数分别为60、80和141，抗虫等位变异较感虫等位变异幼虫重量显著降低了16.4%。
STUDY ON THE RESISTANCE OF SOYBEAN TO SPODOPTERA LITURA BY MOLECULR MARKER ASSISTED SELECTION
ABSTRACT
Spodoptera litura is a leaf eating pest which has a great influence on the yield and quality of soybean. Chemical insecticides are still the main control methods of S. litura. However, chemical control has serious pollution and great impact on food safety, and there are many difficulties in traditional insect resistant breeding. Therefore, effective control of pests and reducing excessive use of pesticides are the trend of soybean pest control. In this study, the method of feeding S. litura in the room was adopted. The leaves of soybean resources collected in the field were used to feed the larvae of S. litura in the room. The molecular markers of resistance to S.litura in F2 population of soybean combination CSSL59 × RIL227 were analyzed by variance analysis and multiple comparison of larval weight of S. litura at the 12th day, the F values of Sat _258 and satt626 were significant, indicating that there was a significant difference in larval weight. There were 24 plants with CSSL59 allele in Sat _258 locus, and the weight of resistant allele was 14.7% lower than that of susceptible allele. The number of plants with CSSL59, LY227 and heterozygous allele in Satt626 was 60, 80 and 141, respectively. The weight of resistant allelic variation was significantly reduced by 16.4% compared with susceptible allele.

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