目录
摘要Ⅱ
关键词Ⅱ
AbstractⅢ
引言
引言（或绪论）1
1 材料与方法2
1.1 试验地概况 2
1.2 实验材料与处理 2
1.3 土壤样品的采集与保存 2
1.4 研究路线 2
1.5 数据处理与分析 3
2 结果与分析3
2.1 土壤氮素营养3
2.1.1 土壤铵态氮3
2.1.2 土壤硝态氮5
2.1.3 土壤全氮7
2.2 土壤酶8
2.2.1 土壤过氧化氢酶9
2.2.2 土壤脲酶10
2.2.3 土壤蔗糖酶12
2.3 土壤氮素营养和酶活性之间的相关分析与回归分析13
2.3.1 增温作用下土壤酶与氮素营养的相关分析与回归分析14
2.3.2 不同施氮量下土壤酶与氮素营养的相关分析与回归分析15
3 讨论 18
3.1 温度和施氮量对土壤氮素营养的影响 18
3.2 温度和施氮量对土壤酶的影响 19
3.3 土壤氮素营养含量和酶活性的关系 19
3.4 不足与展望 19
4 结论 20
致谢20
参考文献20
增温条件下不同施氮量对麦田土壤氮素营养及酶的影响
摘 要
本研究人工模拟气候变暖情景，探讨增温与氮肥对麦田土壤氮素营养以及各种酶的影响。结果表明：与对照组（不增温：W0）相比，增温（W1）处理对土壤铵态氮、全氮含量及过氧化氢酶、脲酶活性没有显著的影响。花前增温减少了土壤硝态氮的含量，抑制了蔗糖酶的活性。施氮处理对土壤中硝态氮以及全氮的含量有重要的影响，N1（225kg/hm2）、N2（375kg/hm2）处理下的土壤全氮以及硝态氮的含量较N0（150kg/hm2）处理下高。在施氮的三个处理中，N1处理增加了土壤铵态氮含量。氮肥处理抑制了冬小麦土壤中过氧化氢酶的活性，增强了蔗糖酶活性以及出苗、拔节、抽穗三个时期的脲酶活性。温度和氮肥互作对冬小麦土壤铵态氮、 *51今日免费论文网|www.51jrft.com +Q: ￥351916072$ 
全氮含量和过氧化氢酶、蔗糖酶活性产生较重要的积极作用。增温降低了土壤铵态氮和脲酶活性的正相关系数；增强了过氧化氢酶的活性与氮素营养含量之间的相关性；使得铵态氮与蔗糖酶之间的抑制作用较W0处理下变得更大。低氮处理更有利于过氧化氢酶的活性的增加；且硝态氮含量与过氧化氢酶之间的相关系数较高；土壤各种酶之间、营养元素之间也有一定的相互影响。
EFFECTS OF DIFFERENT N APPLICATION ON N NUTRIENTS AND ENZYMES IN WHEAT FIELD UNDER WARMING CONDITION
ABSTRACT
Data show that about 35~40 per cent of people feed on wheat. Climate warming has a certain impact on the growth and production of wheat, the unreasonable application of nitrogen fertilizer makes the proportion of soil nutrients out of balance, and a large amount of waste of resources. The effects of warming and nitrogen fertilizer on soil nitrogen nutrition and various enzymes in wheat field were studied. The results showed that compared with the control group (no warming: W0), the warming (W1) treatment had no significant effect on soil ammonium nitrogen, total nitrogen content, catalase and urease activity. preflower warming reduced the content of soil nitrate nitrogen and inhibited the activity of sucrase. Nitrogen treatment of nitrate in soil and the content of total nitrogen had an important effect. the content of total nitrogen and nitrate nitrogen in soil treated with N1(225 kg/hm2), n2(375 kg/hm2) was higher than that of N0(150 kg/hm2). among the three treatments applied to nitrogen, N1 treatment increased soil ammonium nitrogen content. Nitrogen fertilizer treatment inhibited catalase activity in winter wheat soil and enhanced the activity of sucrase and urease in seedling emergence, jointing and heading. The interaction of temperature and nitrogen fertilizer had an important positive effect on ammonium nitrogen, total nitrogen content, catalase and sucrase activity in winter wheat soil. Increased temperature decreased positive correlation coefficient of soil ammonium nitrogen and urease activity; enhanced the correlation between catalase activity and nitrogen nutrient content; and made the inhibition between ammonium nitrogen and sucrase become greater under W0 treatment. Low nitrogen treatment is more beneficial to the increase of catalase activity, and the correlation coefficient between nitrate nitrogen content and catalase is higher, and there is a certain interaction between various enzymes and nutrient elements in soil.

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