目录
摘要Ⅰ
关键词Ⅰ
AbstractⅡ
引言
引言
1材料与方法1
1.1供试果蝇1
1.2果蝇的饲养配方和饲养条件2
1.3供试杀虫剂2
1.4杀虫剂室内毒力生物测定2
2结果与分析3
2.1果蝇 FMO过表达对杀螟丹毒力的影响3
2.2果蝇 FMO过表达对呋虫胺毒力的影响4
3讨论和结论 5
致谢5
参考文献6
黄素单加氧酶与杀虫剂抗性的关系
摘要
黄素单加氧酶（Flavincontaining monooxygenases，FMOs）作为一类重要I相解毒代谢酶，在药物代谢中起着至关重要的作用。前期研究发现甜菜夜蛾抗性种群的FMO基因表达水平与FMO酶活性水平均显著高于敏感种群，说明FMO与杀虫剂代谢以及抗药性相关，但尚缺乏直接证据证明FMO的高表达与抗药性直接相关。本研究拟在前期已构建过表达FMO果蝇的基础上，采用生物测定方法测定FMO过表达果蝇对杀虫剂敏感性的影响。生物测定结果表明，杀螟丹与呋虫胺对果蝇对照品系的LC50值分别为55.022 mg/L和2.710 mg/L，FMO1过表达果蝇对杀螟丹与呋虫胺的LC50值增加为123.533 mg/L和3.437 mg/L，分别提高了2.3和1.3倍；FMO2过表达果蝇对杀螟丹与呋虫胺的LC50值增加为75.551 mg/L和3.262 mg/L，分别提高1.4和1.2倍。研究结果表达FMO的过表达降低了果蝇对这2种杀虫剂的敏感性，说明FMO的过表达会导致对杀虫剂抗性水平的增加，本研究为昆虫FMO介导杀虫剂抗药性提供了直接证据，增进了人们对害虫抗药性机制的了解。
RELATIONSHIP BETWEE FLAVINCONTAINING MONOOXYGENASE AND INSECTICIDE RESISTANCE
ABSTRACT
Flavincontaining monooxygenases (FMOs), as an important class I phase detoxifica *51今日免费论文网|www.51jrft.com +Q: @351916072@ 
tion metabolic enzyme, play a vital role in drug metabolism. Previous studies shown that FMO gene expression levels and FMO enzyme activity levels in the resistant populations of Spodoptera exigua were significantly higher than those in susceptible populations, indicating that FMO is related to insecticide metabolism and resistance, however the direct evidence to associate the FMO overexpression and resistance remains lack. In this study Drosophila melanogaster overexpressing FMO genes which were constructed previously were applied to determine the effect of FMO overexpression on insecticide susceptibilities using the dietincorporation protocol. The bioassay demonstrated that the LC50 value of wild type strain of D. melanogaster were 55.022 mg/L and 2.710 mg/L for cartap and dinotefuran, respectively. FMO1 overexpression significantly decreased the toxicities of these two insecticides, the LC50s were 123.533 mg/L and 3.437 mg/L for cartap and dinotefuran, respectively. The susceptibilities of FMO1 overexpression fruit fly decreased 2.3 and 1.3 times, respectively. Similarly FMO2 overexpression also decreased the toxicities of these two insecticides to fruit fly, the LC50 values increased to 75.551 mg/L and 3.262 mg/L, respectively, the resistance were increased by 1.4 and 1.2 times for cartap and dinotefuran, respectively. These results showed that FMO overexpression reduced the susceptibilities of D. melanogaster to these two insecticides, indicating that FMO overexpression could lead to the increase of insecticide resistance. Our study provides the direct evidence that overexpression of FMO confer insecticide resistance and increase our understand on the mechanism for insecticide resistance.

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