目录
摘要 Ⅰ
关键词 Ⅰ
Abstract Ⅱ
引言
引言
1 材料与方法： 1
1.1 实验材料： 1
1.2 材料处理 1
1.3 实验方法 1
1.3.1 植物材料准备 1
1.3.2 ChIPseq文库构建 1
1.3.3 ChIPseq数据分析 2
2 结果与分析： 3
2.1 H3K4me3比对数据统计 3
2.2 H3K4me3位置分析 4
2.2.1 H3K4me3在基因上位置分析 4
2.2.2 H3K4me3在基因组位置分析 5
2.3 H3K4me3对基因表达影响 6
2.3.1 H3K4me3与基因表达相关性 6
2.3.2 H3K4me3对同源基因偏向影响 7
3 讨论： 8
3.1 组蛋白修饰的位置 8
3.2 组蛋白修饰对高等植物基因表达的作用 8
致谢 9
参考文献 9
解析大豆组蛋白H3K4me3参与基因表达调控作用
摘 要
表观遗传修饰对多倍体物种进化、调控亚基因组间基因表达的不平衡性具有重要作用。组蛋白赖氨酸甲基化在包括植物在内的多细胞真核生物基因表达的表观遗传调控中起着重要作用，在植物中，它塑造植物的发育和生长计划，以及对环境的反应。近年，组蛋白修饰分析技术在植物领域进行了很多相关研究，前人以拟南芥作为模式植物，研究了特定组蛋白修饰的位置和作用机制，随着研究技术发展，学者在小麦和水稻等主要作物中也开展了植物组蛋白修饰研究。通过研究发现，植物中组蛋白修饰在染色体上的分布具有物种特异性，与基因组进化和大小相关。大豆是一种原产于中国的双子叶植物，它是人类重要的蛋白质和油料来源，同时大豆也是主要的固氮作物之一，目前大豆逐渐被应用于表观遗传修饰在多倍体植物基因表达调控中的作用的研究中。本研究利用免疫共沉淀及第二代测序技术，运用生物信息学分析手段，探究了H3K4me3修饰在大豆基因组上的分布和H3K *51今日免费论文网|www.51jrft.com +Q: &351916072& 
4me3与大豆基因表达的相关性。
ANALYSIS OF SOYBEAN HISTONE H3K4me3 INVOLVED IN GENE EXPRESSION REGULATION
ABSTRACT
Epigenetic modification plays a vital role in the evolution of polyploid species and regulating the imbalance of gene expression between subgenomes. Histone lysine methylation exerts a significant influence on the epigenetic regulation of gene expression in multicellular eukaryotes, including plants. In plants, it shapes plant development and growth plans and responses to the environment. In recent years, histone modification analysis technology has carried out many related studies in the field of plants. Previously, Arabidopsis was used as a model plant to study the location and mechanism of specific histone modifications. With the development of research technology, scholars have studied wheat and rice. Plant histone modifications have also been carried out in major crops. Through research, it has been found that the distribution of histone modifications on chromosomes in plants is speciesspecific, which is related to the evolution and the genome size. Soybean is a dicotyledonous plant native to China. It is a momentous source of protein and oil for human beings. Soybean is also one of the main nitrogenfixing crops. Soybean is currently being applied to epigenetic modification in the regulation of gene expression in polyploid plants The role of research. In this study, the use of immunoprecipitation and secondgeneration sequencing technology, using bioinformatics analysis methods, explored the distribution of H3K4me3 modifications on the soybean genome and the correlation between H3K4me3 and soybean gene expression.

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