目录
摘要II
关键词II
AbstractIII
引言
引言1
1 材料与方法2
1.1 实验材料.2
1.2 实验方法.2
1.2.1 找出菎诺藜基因组上全部的NBSLRR基因2
1.2.2 NBSLRR类型基因的分类.....2
1.2.3 菎诺藜NBSLRR类基因的系统发生学树的构建3
1.2.4 菎诺藜 NBSLRR类基因的物理定位...3
1.2.5 特殊蛋白序列的筛选..3
2 结果与分析3
2.1 菎诺藜中含有NBS结构域基因的鉴定和分类3
2.1.1 菎诺藜中含有NBS结构域基因的鉴定.3
2.1.2 菎诺藜中含有NBS结构域基因的分类.....4
2.2 菎诺藜NBS抗病基因中其他的特殊蛋白结构域....5
2.3 菎诺藜NBSLRR抗病基因系统发育树分析...5
2.4 菎诺藜中NBSLRR类型基因的物理定位...6
2.5 特殊蛋白序列筛选结果.....9
3 结论..........11
致谢.........................................11
参考文献..............13
菎诺藜NBSLRR免疫受体全基因组生物信息学分析
摘 要
本研究利用NCBI、PFAM等数据库，MEGA5.1、TBtools、hmmsearch等软件，以及SMART、CDD等网站对菎诺藜（Chenopodium quinoa）的核苷酸结合位点和富含亮氨酸的重复序列（NBSLRR, Nucleotide binding siteleucine rich repeat）类基因家族进行生物信息学鉴定，同时对所属的抗病蛋白类型、基因在染色体上的定位、系统进化关系、蛋白序列中的其他保守功能域等进行分析。我们发现菎诺藜的NBSLRR抗病蛋白共有372个，主要分为五大类型，其中CNL有122个、TNL有2个、NL有88个、CN有69个、N有91个。除 *51今日免费论文网|www.51jrft.com +Q: ^351916072# 
了NBSLRR 主要功能域以外，还发现了一些蛋白具有WRKY、B3、PP2、PRK、RPW等功能域，可能在蛋白参与的对植物的免疫防御过程中起协同作用，调节植物生长发育。这些抗病基因在染色体上分布不均，主要以单基因方式分布，占比71.3%；由于基因簇的存在，可以推测在菎诺藜基因组的进化过程中，有些抗病基因也存在基因复制的现象。
WHOLE GENOME BIOINFORMATICS ANALYSIS OF Chenopodium quinoa NBSLRR IMMUNORECEPTORS
ABSTRACT
In the present study, we used NCBI and PFAM databases, MEGA5.1, TBtools, hmmsearch, SMART, and CDD webservers, to analyze the nucleotide binding sites and leucinerich repeat (NBSLRR) gene families of Chenopodium quinoa, including the types of diseaseresistant proteins, gene localization on chromosomes, and the amino acids of these diseaseresistant proteins. We identified 372 NBSLRR disease resistance proteins in the whole genome of Chenopodium quinoa, including five subgroups, i.e. 122 CNL, 2 TNL, 88 NL, 69 CN and 91 N. In addition to the main functional domains there are also other functional domains such as WRKY, B3, PP2, PRK, RPW, which mainly play a synergistic role in plant immune defense and regulate the growth and development of plant. These diseaseresistant genes are unevenly distributed on chromosomes, mainly in a singlegene manner, accounting for 71.3% of the total, and due to the presence of gene clusters, it can be known that there is also a gene replication process in these diseaseresistant genes during the evolutionary process.
KEY WORDS：Chenopodium quinoa; Bioinformatics; NBSLRR; Resistant gene

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