目录
摘要Ⅱ
关键词Ⅱ
Abstract....III
引言
引言....................................................................................................................1
1 材料与方法.2
1.1 实验材料 ........2
1.1.1 实验菌种.......................................................................................................................2
1.1.2 供试植物......................................................................................................................2
1.1.3 培养基及培养条件........................................................................................................2
1.1.4 实验试剂耗材与仪器.................................................................................................3
1.2 实验方法 ........3
1.2.1 大豆种子的萌发、移栽.....3
1.2.2 疫霉的活化......3
1.2.3 诱导疫霉游动孢子..........4
1.2.4 根瘤菌的活化与转接4
1.2.5 制备稀释待接种菌液4
1.2.6 测量形态学及生理生化相关指标......5
1.2.7 测量叶绿素含量5
1.2.8 测量固氮酶酶活5 *51今日免费论文网|www.51jrft.com +Q: ^351916072* 

2 结果与分析7
2.1 大豆生长情况观察7
2.2 叶绿素含量的测定..7
2.3 叶片数量的测定......8
2.4 株高的测定..............9
2.5 地上部分重量的测定9
2.6 固氮酶活的测定......10
2.7 根瘤数的测定............11
3 分析与讨论 12
致谢13
参考文献14
接种大豆根瘤菌对大豆疫霉致病力影响的研究
摘 要
大豆作为当今世界第五大粮食产物，其农业生产效率受到广泛关注。疫霉是大豆上重要致病菌之一，危害极大，目前主要通过高成本的化学药物进行防控。近年来，利用微生物之间的互作关系来改善宿主植物应对病原菌侵染的生长情况的方法受到广泛的重视。本实验利用大豆为实验材料，通过形态学及生理生化等实验来观察根瘤菌与疫霉在植物体内的相互作用及对各自生理功能的影响。在培养过程中通过观察大豆植株病情指数，我们发现接种大豆根瘤菌能够延缓大豆疫霉的发病时间，减轻大豆疫霉的致病力；通过测量大豆植株叶绿素含量以及地上部分鲜重及干重，我们发现感染疫霉会降低植株光合作用并使光合作用累积产物降低，而接种根瘤菌能够减轻疫霉对植株造成的失绿情况；通过统计叶片数量以及株高发现，感染疫霉会使加速植株叶片脱落并抑制植株生长，接种根瘤菌能够使植株保持较好的生长状态；通过测量根瘤数并计算固氮酶活性，我们发现在感染疫霉的情况下大豆结瘤数量下降，固氮酶活性降低，说明疫霉的侵染也会影响根瘤菌的共生能力。综上所述，根瘤菌与疫霉在大豆植株上的互作会彼此影响，一方面，根瘤菌会降低疫霉的致病力，使植物更好的进行光合作用和生长；另一方面，疫霉也会影响根瘤菌在大豆上的共生，使结瘤固氮受到一定抑制。
STUDY ON THE EFFECT OF INOCULATION OF SOYBEAN RHIZOBIUM ON THE PATHOGENICITY OF PHYTOPHTHORA SOJAE
ABSTRACT
As the fifth largest grain product in the world today, soybean has attracted wide attention for its agricultural production efficiency. Phytophthora soja is one of the important pathogenic bacteria on soybeans, which is extremely harmful. At present, it is mainly controlled by highcost chemical drugs. In recent years, more and more people have focused on improving the growth of host plants in response to pathogen infection by utilizing the interaction between microorganisms. In this experiment, soybean was used as the experimental material to observe the interaction between rhizobium and Phytophthora soja in plants through morphological, physiological and biochemical experiments. During the cultivation process, we found that inoculation of soybean rhizobium can delay the onset of Phytophthora sojae and reduce the pathogenicity of Phytophthora soja, by observing the disease index of soybean plants. By measuring the chlorophyll content of soybean plants and the fresh and dry weights of overground parts, we found that infected Phytophthora sojae would reduce photosynthesis and photosynthetic accumulation products, and inoculation of rhizobium could reduce the chlorosis caused by Phytophthora sojae. Through statistics on the number of leaves and plant height, we found that infected Phytophthora sojae would accelerate the loss of leaves and inhibit the growth of plants, and inoculation of rhizobium could keep the plants in a good growth state. By measuring the number of root nodules and calculating the activity of nitrogenase, we found that the number of nodules decreased and the activity of nitrogenase decreased when infected with Phytophthora sojae, indicating that the infection of Phytophthora sojae would also affect symbiosis. To sum up, the interaction between rhizobium and Phytophthora sojae on soybean plants will affect each other. On the one hand, rhizobia will reduce Phytophthora sojae pathogenicity and make plants better photosynthesize and grow. On the other hand, Phytophthora sojae can also affect the symbiosis of rhizobium on soybean, so that nitrogen fixation of nodules is inhibited to some extent.

原文链接：http://www.jxszl.com/nongxue/zwbh/607380.html