我国是世界上最大的梨树种植面积和产量的国家，在一些地区的梨树产业已经成为了当地的农业经济支柱性产业。如今由于现代自然气候的转变，我国梨树的大部分栽培地区旱情有一些严重，土壤水分的缺少是影响梨树生长发育的主要因素之一，所以提高梨树的生长势和抗旱性，对提高梨树的产量、促进农村经济的发展具有着重要意义，也有利于我国全面小康社会的建设和农村农业现代化的建设。根据前人研究，PbrATL1基因是植物细胞中RING-H2手指蛋白编码的ATL基因家族成员之一，RING-H2手指蛋白广泛的存在于植物中，在许多发育过程的调控以及植物与环境的相互作用中发挥着重要作用。PbrATL1基因可能能够提高一些植物的抗旱性能，但在梨树上还没有进行相关的研究。所以本次研究是对PbrATL1基因的功能进行鉴定，深入了解PbrATL1基因。通过基因工程首先获得具有PbrATL1基因的超表达拟南芥株系，与野生型拟南芥株系同时进行干旱处理，结果发现，发现具有PbrATL1基因的超表达株系比野生型拟南芥株系的脱水率低，电导率低，超氧阴离子含量低，细胞膜完整性好以及三种抗氧化酶（POD、SOD、CAT）的活性都较高，具有PbrATL1基因的超表达株系的抗干旱性能明显提高。以上结果表明，PbrATL1基因能够有效地提高植物的抗旱性能，在植物对非生物胁迫的适应中能够起到重要的作用。
目录
摘要I
关键词I
AbstractI
引言
引言
1 材料与方法1
1.1 植物材料 1
1.2 方法 1
1.2.1 PbrATL1基因的生物信息学分析1
1.2.2 酵母双杂技术1
1.2.3 PbrATL1基因克隆1
1.2.4 中间载体的构建2
1.2.5 农杆菌感受态细胞的制备2
1.2.6 农杆菌感受态细胞的转化2
1.2.7 拟南芥转化2
1.2.8 阳性转基因植物的检测以及超表达系的筛选3
1.2.9 脱水率测定 3
1.2.10 电导率测定3
1.2.11 NBT染色和台盼蓝染色3
1.2.12 过氧化物酶（POD）、超氧化物歧 *51今日免费论文网|www.51jrft.com +Q: ^351916072^ 
化酶（SOD）、过氧化氢酶(CAT）活性测定3
1.2.13 实时荧光定量PCR3
2 实验结果与分析4
2.1 PbrATL1的生物信息学分析4
2.2 PbrATL1基因的克隆、拟南芥转化以及超表达系的筛选5
2.3 干旱胁迫现象5
2.4 干旱相关指标测定7
2.4.1 脱水率测定7
2.4.2 电导率测定7
2.4.3 NBT染色和台盼蓝染色8
2.4.4 过氧化物酶（POD）、超氧化物歧化酶（SOD）、过氧化氢酶(CAT）活性测定8
2.5 PbrATL1基因的调控机制解析9
3 讨论和结论 12
致谢13
参考文献14
梨果实抗旱基因PbrATL1功能初步鉴定
Preliminary Identification Of PbrATL1 Function Of Pear Fruit Drought Resistance Gene
Student majoring in Horticulture Hu Chaoyang
Tutor Huang xiaosan
Abstract： China is the country with the largest pear planting area and yield in the world.Now due to the change of modern natural climate, most of the pear tree planting areas in China there are some serious drought, lack of soil moisture is one of the main factors influencing the pear tree growth, so improve the pear tree growth potential and drought resistance, to improve the production of pear trees, promote the development of rural economy has an important significance, but also to the construction of a welloff society in an allround way in our country and the construction of agricultural modernization in the countryside.According to previous studies, PbrATL1 gene is a member of the ATL gene family encoding the ringh2 finger protein in plant cells. The ringh2 finger protein is widely existed in plants and plays an important role in the regulation of many developmental processes and the interaction between plants and the environment.The PbrATL1 gene may improve drought resistance in some plants, but it has not been studied in pear trees.Therefore, the purpose of this study is to identify the function of PbrATL1 gene and further understand PbrATL1 gene.Through genetic engineering have the first obtain PbrATL1 gene overexpression of arabidopsis strain, with wildtype arabidopsis strain drought treatment at the same time, the results found that found with PbrATL1 gene overexpression strain than wildtype arabidopsis strains of dehydration rate is low, low conductivity, the ultra oxygen anion content is low, the cell membrane integrity is good and three kinds of antioxidant enzyme (POD, SOD, CAT) activity is higher, with PbrATL1 gene overexpression of drought resistance strain has improved significantly.The above results indicated that PbrATL1 gene can effectively improve the drought resistance of plants and play an important role in the adaptation of plants to abiotic stress.

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