目录
摘要Ⅱ
关键词Ⅱ
AbstractⅢ
引言
引言1
1材料与方法2
1.1实验动物2
1.2实验方法2
1.2.1灌喂高浓度葡萄糖急性试验2
1.2.2投喂不同糖水平日粮养殖试验2
1.2.3样品采集3
1.2.4 foxO1基因克隆4
1.2.5核苷酸序列及生物信息学分析4
1.2.6 FoxO1a在不同处理和不同组织中的相对表达量分析4
1.3 数据统计与分析5
2结果与分析5
2.1团头鲂foxO1a的分子克隆和结构分析5
2.2团头鲂foxO1a氨基酸的系统进化分析6
2.3急性高糖灌喂后团头鲂foxO1a在肝脏中的表达特征9
2.4不同碳水化合物水平日粮养殖条件下团头鲂foxO1a的组织表达特征10
3讨论11
小结12
致谢12
参考文献13
团头鲂叉头状转录因子（foxO1）基因序列特征及表达分析
摘 要
FoxO1（forkhead box transcription factor O1）是一种能量代谢的重要调节因子。本研究通过cDNA末端快速扩增（RACE）技术成功克隆出团头鲂（Megalobrama amblycephala）foxO1a mRNA全长序列，应用生物信息学方法对其序列特征进行解析；采用荧光定量PCR技术分析了团头鲂foxO1a的组织分布图谱及高浓度葡萄糖（3g/kg，体重）灌喂条件下团头鲂foxO1a在肝脏中的表达变化。研究结果表明，foxO1开放阅读框（open reading frame, ORF）为1965 bp，编码654个氨基酸；团头鲂foxO1氨基酸序列与草鱼（Ctenopharyngodon idella）的FoxO1氨基酸序列同源性最高，foxO1在肝脏中的表达量最高，其次是肠道和脑，在脾脏和肾脏中的表达峰度较低。不同糖水平（0%, 17%, 34%和51%）日粮对肾和肠中foxO1的表达水平有显著影响；肾foxO1a表达水 *51今日免费论文网|www.51jrft.com +Q: ^351916072* 
平随日粮糖水平的升高呈上升趋势；在肠组织中foxO1表达量随日粮糖水平的升高呈倒“V”形变化，其中17%组foxO1表达量最低，51%组最高。口服高浓度葡萄糖之后1 4 h，团头鲂肝脏中的foxO1a表达水平显著上调。本研究进一步丰富了foxO1在鱼类研究中的基础资料，为鱼类糖代谢调控提供了理论基础。
 GENE SEQUENCE CHARACTERISTICS AND EXPRESSION ANALYSIS OF THE HEADFORK TRANSCRIPTION FACTOR (FOXO1)
ABSTRACT
FoxO1 is an important regulator of energy metabolism. In this study, FoxO1a of Megalobrama amblycephala was successfully cloned by race The tissue distribution map of FoxO1a and the expression of FoxO1a in the liver of Megalobrama amblycephala were analyzed by fluorescence quantitative PCR. The results showed that the open reading frame (ORF) of FoxO1 was 1965 BP, encoding 654 amino acids; the FoxO1 amino acid sequence of Megalobrama amblycephala had the highest homology with that of Ctenopharyngodon idella, the highest expression of FoxO1 in liver, the second in intestine and brain, and the lowest expression peak in spleen and kidney. Different dietary sugar levels (0%, 17%, 34% and 51%) had significant effects on the expression of FoxO1 in kidney and intestine; the expression level of FoxO1a in kidney increased with the increase of dietary sugar level; the expression level of FoxO1 in intestine changed in an inverted "V" shape with the increase of dietary sugar level, in which the expression level of FoxO1 in 17% group was the lowest and that in 51% group was the highest. The expression level of FoxO1a in the liver of Megalobrama amblycephala was significantly increased 1 4 hours after oral administration of high concentration glucose. This study further enriched the basic data of FoxO1 in fish research, and provided a theoretical basis for the regulation of fish sugar metabolism.

原文链接：http://www.jxszl.com/nongxue/scyz/606700.html