目录
摘 要 II
关键词 II
Abstract III
引言
引言 1
1 材料与方法 7
1．1 实验试剂及仪器 7
1．2 技术路线 8
1．3 合成磁性材料 8
1．4 构建可循环纳米荧光探针 8
1．5 检测阿特拉津 8
1．6 离子干扰性验证 9
2．1 磁性材料的结构及其性质的表征 9
2.1.1 形貌分析 9
2.1.2 磁性纳米粒子的磁性验证 9
2.1.3 磁性纳米材料的紫外光谱 10
2.2 凝胶电泳实验 10
2.3 探针检测阿特拉津 11
2.4 探针检测阿特拉津抗离子干扰性验证 12
3 讨论 13
致谢 13
参考文献 13
基于可循环磁性纳米探针的农药荧光传感器的研究
摘 要
在现代农业实践中，农药主要通过消杀害虫及除草来促进农业生产力的提高与发展。但是，土壤中的农药残留不仅严重污染了食物，此外还严重破坏了生态系统，给社会造成了极大的危害，甚至影响了人们的日常生活。其中，北方旱区作物的常用除草剂—阿特拉津，是一种人工合成的三嗪苯类除草剂。主要适用于玉米、高粱、果园和林地等，其使用大大促进了世界精耕农业和效益农业的发展。虽然阿特拉津的毒性比较低，但研究表明20%~70%阿特拉津可以在土壤中存留很长时间，并且结构稳定，在水中溶解度高，不容易被降解。近年来，世界各国高度重视阿特拉津引起的大范围污染、难以降解和扰乱内分泌等环境与健康问题。本课题通过合成磁性材料并对其进行功能化，通过测试其荧光性能的变化，构建磁性阿特拉津检测传感器。该传感器可对阿特拉津实现“关开”荧光信号模式转变，并通过验证该传感器检测阿特拉津的特异性，得到一种基于适配体的快速简便、灵敏度高的阿特拉津荧光检测技术，并有望应用于可循环探针的开发及利用，推动农药荧光传感器的发展。
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R FOR PESTICIDE DETECTION
ABSTRACT
In the practice of modern agriculture, pesticides promote the improvement and development of agricultural production mainly by killing pests and controlling weeds. However, the residue of pesticide in the soil not only contaminates the crop seriously, but also damages the ecosystem seriously, which brings great harm to the society and affects the people’s daily life. Among the pesticides, atrazine as a synthetic triazine herbicide is widely used for the crops including corn, sorghum, fruit and forest in the northern arid region of China. The use of atrazine has greatly promoted the development of intensive farming and efficient agriculture in the world. Although regarded as a low toxic herbicide, 20%~70% of atrazine was confirmed to be difficult to degrade and remain in the soil for a long time in the application process due to its stable structure, strong water solubility and long halflife period according to the latest reports. In these years, the environmental and health problems caused by atrazine’s global pollution, environmental persistence and endocrine interference have attracted the attention of all countries in the world. In this project, through the synthesis and functionalization of magnetic materials, a magnetic atrazine sensor was designed and constructed by monitoring the fluorescence performance and response against atrazine. The nanoscale sensor realized the “offon”fluorescence signal transition to atrazine. Moreover, by verifying the specificity to detect atrazine, a rapid, simple but sensitive fluorescent protocol based on the aptamer was developed for atrazine detection, displaying the potential as the recycled nanoprobe for making a contribution to the evolvement of fluorescent pesticide sensors.

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