目录
摘要 Ⅱ
关键词 Ⅱ
ABSTRACT Ⅲ
KEY WORDS Ⅲ
1 引言 1
1.1饮食对肠道菌群影响的相关研究 2
1.2研究目的与意义 4
1.3研究内容与路线 4
2 材料与方法 5
2.1非人灵长类动物粪便样品的收集 5
2.2基因组DNA的提取 7
2.3基因的扩增 7
2.4 PCR产物的混合与纯化 7
2.5文库制备与测序 7
2.6数据的分析 7
2.7血液取样与准备 8
2.8血常规及生化分析 8
2.9统计分析 8
3 结果与分析 9
3.1 OTU聚类统计结果与分析 9
3.2菌群的结构结果与分析 9
3.3菌群α多样性的结果与分析 10
3.4菌群β多样性的结果与分析 11
3.5肠道菌群LEfSe的结果与分析 12
3.6血常规与血生化指标的比较 13
4 讨论 19
致谢 22
参考文献 23
饮食对非人灵长类动物肠道菌群的影响
摘要
越来越多的研究表明,人类和动物疾病的发病率与饮食习惯相关。饮食习惯的差异性往往会影响宿主身体里营养素的代谢与肠道菌群的平衡,从而导致疾病的发生。非人灵长类动物（NHPs)作为人类近亲,是研究饮食习惯对肠道微生物变化的理想模型。本研究通过16SrRNA基因高通量测序技术,分析了食果类灵长类动物,食叶类灵长类动物和杂食类灵长类动物三类食性不同的非人灵长类动物肠道菌群的组成。研究结果表明,虽饲喂不同饲料的NHPs虽有其独特的特征菌群,但厚壁菌门、拟杆菌门和变形杆菌门为灵长类动物基本的主要菌群。食果类和食叶类灵长类动物肠道微生物群落多样性明显高于杂食类灵长类动物,预示植物纤维摄入量越多肠道微生物多样性越高。同时,不同的饮食习惯同样影响血常规和血液生化指标。本研究初步揭示了肠道菌群与宿主的关系是相互作用和长期进化的结果,饮食习惯不同的动物其肠道菌群存在区别,与其营养代 *51今日免费论文网|www.51jrft.com +Q: @351916072@ 
谢和疾病发生存在关联,为今后健康饮食提供立论依据。
关键词 饮食习惯；非人灵长类动物；肠道菌群；肠道健康
EFFECTS OF FOOD ON INTESTINAL MICROFLORA IN NONHUMAN PRIMATES
ABSTRACT
More and more studies have shown that the incidence rate of human and animal diseases is related to their eating habits. The difference of eating habits often affects the balance of nutrients metabolism and intestinal flora in the host body, which leads to the occurrence of diseases. Non human primates (NHPs), as close relatives of human beings, are ideal models to study the effect of dietary habits on intestinal microbial changes. In this study, the composition of intestinal microflora of nonhuman primates with different feeding habits was analyzed by highthroughput sequencing of 16SrRNA Gene. The results showed that although the NHPs fed with different feeds had common characteristic flora, the phylum Pachytene, Bacteroidea and Proteus were the main flora of primates. The diversity of intestinal microflora in fruit eating and leaf eating primates was significantly higher than that in omnivorous primates, indicating that the higher intake of plant fiber, the higher diversity of intestinal microflora. At the same time, different eating habits also affect blood routine and blood biochemical indicators. This study preliminarily revealed that the relationship between intestinal flora and host is the result of interaction and longterm evolution. There are differences in intestinal flora of animals with different dietary habits, which are related to their nutritional metabolism and disease occurrence, providing a theoretical basis for healthy diet in the future.

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