目录
摘要Ⅲ
关键词Ⅲ
AbstractⅥ
引言
引言1
1 文献综述1
1.1 病原1
1.1.1 支气管败血波氏杆菌1
1.1.2 多杀性巴氏杆菌2
1.2 致病机理2
1.3 临床症状3
1.4 流行病学3
1.5 诊断3
1.6 防治4
1.7 疫苗4
2 材料与方法 4
2.1 材料4
2.1.1 动物4
2.1.2 试剂4
2.1.3 仪器5
2.2 方法5
2.2.1 支气管败血波氏杆菌和多杀性巴氏杆菌的鉴定5
2.2.2 攻毒动物的准备6
2.2.3 动物的攻读及病变评分8
3 结果与分析 9
3.1 支气管败血波氏杆菌和产毒素性多杀性巴氏杆菌的鉴定9
3.1.1 菌种形态鉴定9
3.1.2 菌种PCR鉴定9
3.1.3 菌种的培养特性检测10
3.2 攻毒动物的准备11
3.2.1 血清抗体检测11
3.2.2 血清PCR检测11
3.3 动物的攻毒及观察11
4 讨论13
致谢13
参考文献14
图31 油镜下支气管败血波氏杆菌和产毒素型多杀性巴氏杆菌的形态9图32 菌种PCR鉴定9
图33 Bb和Pm的生长曲线及OD600与菌落数的关系 10
图34 实验动物PCR鉴定11
图35 猪的剖检12
图36 仔猪的相对日增重13
表21 多杀性巴氏杆菌和波氏杆菌鉴定引物6
表22 实验猪的攻毒剂量8
表23 萎缩性鼻炎的病变评级8
表31 仔猪鼻甲骨萎缩病变评级12
猪传染性萎缩性鼻炎攻毒模型的建立
摘 要
猪传染性萎缩性鼻炎是由支气管败血波氏杆菌和产毒素型多杀性 *51今日免费论文网|www.51jrft.com +Q: *351916072* 
巴氏杆菌联合感染引起的一种慢性呼吸道传染病。本病主要引起仔猪鼻炎、鼻泪管阻塞、鼻梁变形及鼻甲骨萎缩（主要是下卷曲）等症状。病猪饲料转化率低、生长缓慢，给生产效益带来巨大损失。因此，为了给病理研究和疫苗评价提供一个实用性强的疾病动物模型，本次实验用普通外三元仔猪和大学农业部动物细菌学重点实验室提供的AHBb和ZXT+Pm菌种，通过滴鼻感染的方式，成功建立出猪传染性萎缩性鼻炎攻毒模型。实验设置对照组、攻毒组Ⅰ、攻毒组Ⅱ三个组别，每组五头阴性筛选猪。攻毒组每只猪每个鼻孔先接种0.5mL 8.2×106 CFU/mL的Bb。一周后，攻毒组Ⅰ每头猪每个鼻孔滴鼻接种0.5mL 1×1010 CFU/mL的Pm，连续接种4天；攻毒组Ⅱ每头猪每个鼻孔滴鼻接种0.5mL 1×109 CFU/mL的Pm，连续接种4天。对照组接种程序同上，接种液体换为无菌PBS。21天后剖杀，对照组未发病，攻毒组发病率为100%，重症发病率大于80%。此模型具有发病率高、发病时间短、特征症状明显、动物死亡率低、取材方便、价格经济、贴近临床实际等优势，可以用于实验研究。
ESTABLISHMENT OF A PATHOGENIC MODEL FOR PROGERSSIVE ATROPHIC RHINITIS IN PIGS
ABSTRACT
Infectious atrophic rhinitis of pigs is a chronic respiratory infection caused by the combination of Bordetella bronchiseptica and Pasteurella multocida. This infection mainly causes rhinitis, nasolacrimal duct obstruction, nasal bridge deformation, turbinate bone atrophy (mainly under the crimp) and other symptoms in pigs. The growth and the feed conversion rate of sick pigs is low, which brings huge loss to the swine production. Therefore, in order to provide a suitable animal model of disease for pathological research and vaccine evaluation, commercial piglets and AHBb strains and ZXT+ Pm strains, provided by the Nanjing Agricultural University, were used to establish a pig infectious atrophic rhinitis infection model with the way of intranasal infection. Fifteen pigs were randomly divided into 3 groups and each with 5 pigs. Firstly, 0.5mL 8.2×106 CFU/mL of Bb was inoculated into each nostril of each pig in the inoculation group. A week later, 0.5 mL 1×1010 CFU/mL of Pm was inoculated into each nostril of each pig in the inoculation group I and last for 4 days. In addition, 0.5 mL 1 x 109 CFU/mL of Pm was inoculated into each nostril of each pig in the inoculation group II and last for 4 days.The inoculation procedure of the control group was the same as above, but the inoculation liquid was changed to sterile PBS. After 21 days, there was no symptoms in the control group. The morbidity in the inoculation group was 100%, and the severe status was more than 80%. This model has the advantages of high morbidity, short onset time, obvious characteristic symptoms, low animal mortality, convenient sampling, economical price and close to clinical practice, and can be used for experimental research.

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