目录
目录
摘要Ⅱ
关键词Ⅱ
AbstractⅢ
引言
1.绪论1
1.1H9N2禽流感病毒 1
1.1.1流感病毒的结构1
1.1.2流感病毒的感染机制1
1.1.3 H9N2流感病毒的影响1
1.2反向遗传技术 2
1.2.1依赖于辅助病毒的反向遗传系统 2
1.2.2不依赖于辅助病毒的反向遗传系统3
1.3流感疫苗 3
1.3.1灭活疫苗3
1.3.2减毒活疫苗3
1.3.3基因重组亚单位疫苗4
1.3.4基因重组活载体疫苗4
1.3.5 DNA疫苗4
1.3.6 mRNA疫苗4
2．H9N2亚型禽流感毒株的拯救4
2.1材料5
2.2方法5
2.3结果6
3．反向遗传技术拯救毒株制作疫苗效价对比 7
3.1灭活苗7
3.1.1商品化灭活苗7
3.1.2拯救的灭活苗8
3.1.3拯救灭活苗免疫效果初步评价结果8
3.2截短活疫苗9
3.2.1截短活疫苗原理9
3.2.1截短活疫苗免疫评价9
4．讨论 10
致谢11
参考文献11
H9N2亚型禽流感毒株反向遗传系统的建立及其在疫苗中的应用
摘要
禽流感病毒属于正黏病毒科流感病毒属，是有包膜的单股负链分节段RNA病毒。流感病毒会引起季节性流行或大流行，导致严重的经济以及公共卫生问题,最有效的预防流感病毒的方法就是接种疫苗。在低致病性禽流感疫情中，主要以H9N2亚型流感病毒的危害为主，近年来，需要辅助病毒到完全以质粒为基础的反向遗传学技术的建立和发展，极大地降低了cDNA克隆人工合成流感病毒的难度和技术障碍，并逐渐成为研究流感病毒及生产流感疫苗的重要基础和手段。
在如此众多的H9N2流感疫苗类型中，免疫效果参差不齐，本文先通过用反向遗传学构建一株病毒（DH104），然 *51今日免费论文网|www.51jrft.com +Q: ￥351916072$ 
后构建反向遗传学构建的全病毒灭活株（rDH104）和NS1基因截短株（rTXNS1128），通过对其制作的疫苗进行血清学监测，以及和商品苗的免疫效价对比，可以明显看出利用反向遗传学技术拯救病毒得到的疫苗免疫效果良好。进一步阐述了反向遗传学在拯救H9N2毒株具有良好的疫苗潜力。
ESTABLISHMENT OF REVERSE GENETIC SYSTEM OF H9N2 SUBTYPE AVIAN INFLUENZA STRAIN AND ITS APPLICATION IN VACCINE
ABSTRACT
The avian influenza virus belongs to the genus Influenza virus of the Orthomyxoviridae family, which is an enveloped singlestrand negativestrand RNA virus. Influenza viruses can cause seasonal epidemics or pandemics, leading to serious economic and public health problems. The most effective way to prevent influenza viruses is to vaccinate them. In the outbreak of lowpathogenic avian influenza, the main hazard is H9N2 subtype influenza virus. In recent years, the establishment and development of reverse genetics technology that requires auxiliary viruses to completely based on plasmids has greatly reduced cDNA. The difficulty and technical obstacles of cloning artificially synthesized influenza virus have gradually become an important basis and means for studying influenza virus and producing influenza vaccine.
Among so many types of H9N2 influenza vaccines, the immune effect is uneven. In this paper, we first construct a virus (DH104) by reverse genetics, and then construct a reverse virus genetically constructed inactivated whole virus (rDH104) and NS1 The truncated gene strain (rTXNS1128), through serological monitoring of the vaccine prepared by it and comparison of the immunopotency with commercial vaccines, it can be clearly seen that the immune effect of the vaccine obtained by using reverse genetics to rescue the virus good. Further elaborated that reverse genetics has a good vaccine potential in rescue H9N2 strain.KEY WORDS： Avian influenza; reverse genetic technology; vaccine; immunization evaluation

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