目录
摘要II
关键词II
AbstractIII
引言
引言1
1 材料与方法4
1.1材料4
1.1.1数据收集4
1.1.2生物软件4
1.2方法4
1.3 方案论证5
2 结果分析6
2.1基因组系统进化树的遗传进化分析以及同源性分析6
2.1.1全基因组系统进化树分析6
2.1.2 全基因组同源性分析7
2.2 S蛋白系统进化树的遗传进化分析以及同源性分析10
2.2.1 S蛋白系统进化树分析10
2.2.2 S蛋白同源性分析10
2.3 N蛋白系统进化树的遗传进化分析以及同源性分析11
2.3.1 S蛋白系统进化树分析12
2.3.2 S蛋白同源性分析12
3 讨论与结论14
3.1讨论14
3.2结论16
致谢16
参考文献17
新型冠状病毒(SARSCoV2)与常见动物冠状病毒的遗传变异分析
摘 要
2019年12月，湖北省武汉市首次发现新型冠状病毒(SARSCoV2)感染病例。该病毒感染范围广、传播速度快，目前已波及至全球各国。为了探究新型冠状病毒来源，寻找其中间宿主，本研究从全基因组、S蛋白和N蛋白水平出发，分析了新型冠状病毒和多种常见动物冠状病毒的同源性与遗传进化特征。结果显示，除蝙蝠源冠状病毒外，新型冠状病毒与动物冠状病毒的基因组同源性约47.31%56.78%；新型冠状病毒与动物冠状病毒的S蛋白核苷酸同源性约33.63%47.00%；新型冠状病毒与动物冠状病毒的N蛋白核苷酸同源性约38.62%48.60%。初步推测，新型冠状病毒与常见动物冠状病毒的全基因组、S蛋白和N蛋白同源性偏低，亲缘关系较远；与蝙蝠和SRSA病毒同源性较高，亲缘关系较近。根据研究结果，可初步排除新型冠状病毒从动物冠状病毒演化而来的可能，由此推测新型冠状病毒通过常见动物传播到人的可能性较低。本研究为冠状病毒跨种传播研究提供了参考基础。
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tic Variation Analysis of the Severe Acute Respiratory Syndrome Coronavirus 2 and Animal Coronavirus
ABSTRACT
In December 2019, Wuhan City, Hubei Province, for the first time, had been detected multiple infected cases of the 2019 new coronavirus (SARSCoV2), which spread quickly and caused a wide range of infections around the world. In order to explore the source of the SARSCoV2 and find the intermediate host for its transmission, our study analyzed the homology and genetic evolution characteristics between the SARSCoV2 and various common animal coronaviruses based on the level of complete genome, S protein and N protein. The results show that, except for batderived coronaviruses, the SARSCoV2 has a genomic homology range from 47.31% to 56.78% with common animal coronaviruses; S protein nucleotide homology between SARSCoV2 and common animal coronavirus ranges from 33.63% to 47.00%; SARSCoV2 and common animal coronavirus have a N protein nucleotide homology ranging from 38.62% to 48.60%. It is preliminarily speculated that the SARSCoV2 has a low homology based on the level of complete genome, S protein and N protein with common animal coronaviruses. Therefore, SARSCoV2 has a far genetic relationship with common animal coronaviruses. In addition, the homology within batderived coronaviruses and SRSA virus is higher than other animal coronaviruses. Thus, their genetic relationship is closer. To sum up, we can remove the possibility that the SARSCoV2 evolved from animal coronavirus and speculate that the SARSCoV2 is less likely to spread to humans through common animals. This study provides basic reference for the study of coronavirus crossspecies transmission.

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