目录
摘要II关键词II
AbstractIII
引言
引言1
1 材料与方法3
1.1 耐铬菌株的分离、纯化 3
1.2 菌株耐性固体平板实验 4
1.3 菌株生长曲线测定4
1.4 菌株基因鉴定4
1.5 铬耐性及还原菌促生性质研究4
1.6 目的菌株抗病原菌能力研究5
1.7 目的菌株对植株生长、生理的影响5
1.8 目的菌株对植物吸收铬及根际土壤中铬有效态的影响5
2 结果与分析6
2.1 菌株形态观察6
2.2 基于16S rRNA基因序列对菌株的鉴定7
2.3 菌株耐性固体研究9
2.4 铬胁迫下的菌株生长曲线10
3 讨论11
致谢14
参考文献15
耐铬菌株筛选及铬耐性比较
摘 要
近年，随着经济的发展，我国也出现了各种各样的环境问题，如土地污染等，我国的污染类型以重金属污染为主。随着涉及重金属的行业的迅速发展及环境保护机制的不完善，我国的重金属污染愈发严重，影响农业生产及人民的身体健康。生物修复是新晋的应对手段之一,生物修复可分为微生物修复、植物修复和植物微生物联合修复。生物修复具有成本低，易操作等优点。重金属铬的地球化学循环中，有涉及微生物及植物等的有机环节，所以生物修复可作为治理重金属铬污染的手段。植物与微生物修复分别具有其局限性。微生物难以将重金属从环境中去除，主要作用为将重金属价态转化以达到将其降毒的目的；超富集植物可以富集环境中的重金属，但在污染严重的环境中难以生长，进而影响其富集效果。本实验以微生物为入手点，首先筛选耐铬菌种，研究其对于铬污染的耐性及转化情况，进而确定其对重金属铬污染的修复能力，以及对超富集植物是否具有相关促生作用。
SCREENING OF CHROMIUM RESISTANT STRAINS AND COMPARISON OF CHROMIUM TOLERANCE
ABSTRACT
Recent years, with the  *51今日免费论文网|www.51jrft.com +Q: *351916072* 
development of economy, there are also a variety of environmental problems in China, such as land pollution. Because of the rapid development of heavy metal industry and the imperfection of environmental protection mechanism, heavy metal pollution in China is becoming more and more serious, which affects agricultural production and peoples health. Bioremediation is one new strategies. Bioremediation can be divided into microbial remediation, plant remediation and plant microbial joint remediation. Bioremediation has the advantages of low cost and easy operation. In the geochemical cycle of heavy metal chromium, there are organic links involving microorganisms and plants, so bioremediation can be used as a means to control heavy metal chromium pollution. Phytoremediation and microbial remediation have their own limitations. It is difficult for microorganisms to remove heavy metals from the environment, the main role of which is to transform the valence state of heavy metals to achieve the purpose of reducing their toxicity; Hyperaccumulator can enrich heavy metals in the environment, but it is difficult to grow in the environment with serious pollution, thus affecting their enrichment effect. In this experiment, microorganisms were used as the starting point. Firstly, chromium tolerant bacteria were screened to study their tolerance and transformation to chromium pollution, and then to determine their remediation ability to heavy metal chromium pollution, and whether they have related growth promoting effect on hyperaccumulator

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