纳米释氢气材料ab@hmsn诱导黄瓜不定根发生的初步探究【字数:8225】
目录
摘要II
关键词II
AbstractIII
引言
引言(或绪论)1
1 材料与方法2
材料 2
1.1.1 植物材料2
1.1.2 纳米材料2
1.1.3 主要试剂2
1.2 方法 2
1.2.1 处理2
1.2.2 黄瓜不定根的测量和不定根原基的计数3
1.2.3 统计分析3
2 结果与分析3
2.1 AB@hMSN以浓度依赖的方式诱导黄瓜不定根的发生3
2.2 AB@hMSN诱导不定根发生的有效效应因子是氢气6
3 讨论 8
3.1 纳米材料对植物生长发育的影响 8
3.2纳米材料在氢农学的应用9
致谢11
参考文献12
纳米释氢材料AB@hMSN诱导黄瓜不定根发生的初步探究
摘 要
黄瓜是一种在中国被广泛种植栽培的蔬菜,具有丰富的营养成分。作为气体信号分子之一,氢气(Hydrogen gas, H2)对黄瓜的生长起到一定的调控促进作用。受氢医学领域纳米释氢材料实现肿瘤内氢气高负荷输送的启发,本论文将负氨硼烷分子的中空介孔二氧化硅纳米颗粒(ammonia boraneloaded hollow mesoporous silica nanoparticle, AB@hMSN)应用于露丰黄瓜的生长,通过植物生理学和解剖学等方法,从不定根根长、数目及不定根原基的发生等角度探究AB@hMSN对黄瓜不定根发生的影响。结果显示AB@hMSN对黄瓜不定根发生具有显著促进作用,实验筛选出了该纳米材料促进植物不定根发生的最佳浓度为1 mg/L,明确诱导不定根发生的有效效应因子是纳米材料所负载的氢气,同时也再次验证了氢气对于植株生长的促进作用。该研究利用现在新兴的氢农学技术,对提升黄瓜产量及品质及实现高效便捷的现场氢气管理具有重要意义。由于该纳米材料具有超高的氢气负载量和一定程度的生物安全性,基于纳米材料学的给氢策略可以为氢气在农业上的高效利用提供初步的理论依据。
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MINARY STUDY ON THE OCCURRENCE OF ADVENTITIOUS ROOTS IN CUCUMBER INDUCED BY NANOHYDROGEN RELEASING MATERIAL AB@HMSN
ABSTRACT
Cucumber is a vegetable that is widely cultivated in China and has rich nutrients. As one of the gas signal molecules, hydrogen gas (H2) plays a certain role in regulating and promoting cucumber growth. Inspired by hydrogenreleasing nanomaterials in the field of hydrogen medicine to achieve highload delivery of hydrogen in tumors, this paper applies the application of ammonia boraneloaded hollow mesoporous silica nanoparticle (AB@hMSN) For the growth of Lufeng cucumber, through the methods of plant physiology and anatomy, the influence of AB@hMSN on the occurrence of cucumber adventitious roots was explored from the perspectives of adventitious root length, number and occurrence of adventitious root primordia. The results show that AB@hMSN has a significant promoting effect on cucumber adventitious roots. The optimal concentration of the nanomaterials to promote plant adventitious roots is 1 mg / L. The effective effect factor for inducing adventitious roots is hydrogen which loaded by the nanomaterials. At the same time, it also verified the promotion effect of hydrogen on plant growth. This research uses the newly emerging hydrogen agronomy technology, which is of great significance for improving cucumber yield and quality and achieving efficient and convenient onsite hydrogen management. Due to the ultrahigh hydrogen loading and a certain degree of biological safety of the nanomaterials, the hydrogen supply strategy based on nanomaterials can provide a preliminary theoretical basis for the efficient use of hydrogen in agriculture.
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