目录
摘要 I
关键词 I
ABSTRACT II
KEY WORDS II
引言
1 材料与方法 1
1．1 实验材料 1
1．1．1 试供材料 1
1．1．2 实验试剂 1
1．1．3 主要实验仪器 1
1．1．4 实验常用培养基 1
1．2 实验方法 2
1．2．1 水稻的水培 2
1．2．2 花生的水培 2
1．2．3 小麦的水培 2
1．2．4 大豆的水培 2
1．2．5 叶绿素含量的测定 3
2 结果与分析 4
2．1 外源NE处理对水稻耐盐性的影响 4
2．2 外源NE处理对花生耐盐性的影响 5
2．3 外源NE处理对小麦耐盐性的影响 5
2．4 外源NE处理对大豆耐盐性的影响 6
3 讨论与结论 7
3．1 NE参与农作物NaCl胁迫的耐性调控 7
3．2 关于NE功能和应用的未来展望 8
致谢 8
参考文献 11
去甲肾上腺素调控农作物耐盐性的研究
摘要
儿茶酚胺（Catecholamines, CAs）是具有3, 4二羟基取代苯环的一组生物胺，广泛存在于哺乳动物中，是一种含儿茶酚和氨基的神经递质。该组物质包括多巴胺（Dopamine, DA）、去甲肾上腺素（Norepinephrine, NE）、肾上腺素（Epinephrine, E）及其衍生物。目前CAs在许多植物中也有发现，已有研究表明NE对于拟南芥的根系、气孔等皆存在一定的调控作用。为了进一步探究在NaCl胁迫条件下NE对提高农作物抗逆性的作用，我们以水稻、花生、小麦和大豆四种农作物的幼苗为实验材料，采用水培的方法，研究了NE对NaCl胁迫下农作物的株高、根长、鲜重及叶绿素含量四项生理指标的影响。结果显示，NaCl胁迫会显著抑制水稻、花生和小麦的生长，但对大豆的影响不大，推测可能是由于大豆为了减少NaCl胁迫对于自身生长发育 *51今日免费论文网|www.51jrft.com +Q: ^351916072^ 
所造成的伤害，通过自身机制来维持细胞质的低盐水平。经NE处理后的水稻幼苗，其四项生理指标都有所增加，说明NE能够提高水稻的耐盐性；NE处理花生后，其生理指标均显著增加，说明NE对于提高花生的耐盐性有着非常显著的效果；NE处理小麦和大豆后，两者耐盐性也有所增加，但总体效果不明显。上述结果表明，NaCl胁迫会抑制农作物的生长发育，而一定浓度的NE对于提高农作物的耐盐性，增强农作物的抗逆性具有促进作用。
STUDIES ON THE REGULATION OF NOREPINEPHRINE ON CROPS SALT TOLERANCE
ABSTRACT
Catecholamines (CAs) are a group of biogenic amines with a 3, 4dihydroxysubstituted phenyl ring, which are widely present in mammals and are neurotransmitters containing catechol and amine groups. This group of substances includes dopamine (DA), norepinephrine (NE), epinephrine (E) and their derivatives. At present, CAs are also found in many plants. Studies have shown that NE has a certain regulatory effect on the roots and stomata of Arabidopsis thaliana. In order to further explore the effect of NE on improving the stress resistance of crops under NaCl stress, we used the seedlings of rice, peanut, wheat and soybean as experimental materials and used hydroponic method to research the effects of NE on the four physiological indicators of plant height, root length, fresh weight and chlorophyll content. The results showed that NaCl stress could significantly inhibit the growth of rice, peanut and wheat, but it had little effect on soybean. It might be in order to reduce the damage caused by NaCl stress to its own growth and development, soybean maintained a low salt level of cytoplasm through its own mechanism. The four physiological indicators of rice seedlings treated with NE had increased, indicating that NE could improve the salt tolerance of rice; after NE treatment of peanuts, their physiological indicators had increased significantly, indicating that NE had a very significant effect on improving the salt tolerance of peanuts; after NE treated wheat and soybean, the salt tolerance of both had also increased, but the overall effect was not obvious. These results illustrated that NaCl stress could inhibit the growth and development of crops, and a certain concentration of NE could improve the salt tolerance and enhance the stress resistance of crops.

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