目录
摘要 II
关键词 II
Abstract III
引言
引言 11
1 材料与方法 2
1．1 试验材料 2
1．2 试验设计 3
1．3 试验方法 3
1．3．1 选种与播种 3
1．3．2 配制营养液 3
1．3．3 移苗 3
1．3．4 盐（NaCl）处理 3
1．4 测定指标与方法 3
1．5 表型数据分析 4
1．6 极端材料的筛选 4
1．7 表型数据分析 4
2 结果与分析 4
2．1 苗期表型数据分析 4
2．2 突变体苗期相关性分析 6
2．3 极端材料的筛选 7
2．4 与大豆突变体苗期表型性状相关的SNP标记 8
3 讨论 11
3．1 突变体群体的使用 11
3．2 关联分析与SNP定位 12
致谢 12
参考文献 12
盐胁迫下大豆突变体群体苗期地下部相关
性状的关联分析
摘 要
盐胁迫是大豆生长过程中限制其产量和品质提高的重要非生物逆境，大豆苗期是大豆品质和产量形成的关键时期，也是对盐胁迫最敏感的时期。大豆突变体的使用能够拓宽遗传变异的背景，扩大选择利用的范围。本研究使用由南农“864”和“南农9416”理化诱变而来的132个突变体材料，在温室内用水培法对大豆苗期地下部性状进行耐盐性鉴定，亲本和突变体的10个苗期相关性状在0mM NaCl（CK）或150 mM NaCl（S）间均有极显著差异；除地下部长度以外，其他9个指标间均呈显著或极显著的正相关（个别指标间除外）。根据所有突变体10个性状的U值，筛选出了15个耐盐性较强的突变体材料。结合突变体群体的SNP分子标记对10个性状进行全基因组关联分析，共检测到411个与地下部性状耐盐性显著相关的SNP标记，在16号染色体上检测到了91个；且有113个SNP标记与苗期地下部多个性状显著关联，1号染色体上的 *51今日免费论文网|www.51jrft.com +Q: @351916072@ 
AX93670119标记与8个性状显著关联。该研究为拓宽大豆种质资源和大豆苗期耐盐基因的定位和挖掘提供一定的参考价值。
CORRELATION ANALYSIS OF UNDERGROUND RELATED TRAITS OF SOYBEAN MUTANT POPULATION UNDER SALT STRESS
ABSTRACT
Salt stress is an important abiotic stress that limits soybean yield and quality improvement during soybean growth. Soybean seedling stage is a critical period for the formation of soybean quality and yield, and it is also the most sensitive period to salt stress. The use of soybean mutants can broaden the background of genetic variation and expand the range of selection and utilization. In this study, 132 mutant materials mutated from Nannong "864" and "Nannong 9416" were used to identify the salt tolerance of soybean seedlings under water in the greenhouse. The 10 seedlingrelated traits of the parents and mutants were very significantly different between 0 mM NaCl (CK) or 150 mM NaCl (S); except for the length of the ground, all other 9 indicators showed significant or extremely significant positive Related (except for individual indicators). Based on the U values ​​of 10 mutant traits for all mutants, 15 mutant materials with strong salt tolerance were selected. Combined with the SNP molecular markers of the mutant population, a genomewide association analysis was performed on 10 traits. A total of 411 SNP markers that were significantly related to the salt tolerance of underground traits were detected, and 91 were detected on chromosome 16; and 113 The SNP marker was significantly associated with multiple traits at the seedling stage, and the AX93670119 marker on chromosome 1 was significantly associated with eight traits. This study provides a certain reference value for broadening the location and excavation of soybean germplasm resources and salttolerance genes in soybean seedling stage.

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