目录
摘要Ⅱ
关键词Ⅱ
AbstractⅢ
引言
引言
1材料与方法1
1.1实验材料 1
1.2主要试剂 1
1.3基因的克隆 1
1.4构建BiFC融合表达载体5
2结果与分析7
2.1大豆疫霉菌丝基因组DNA 的提取及大豆叶片RNA的提取7
2.2大豆疫霉效应因子基因PsAvh23及大豆协调冷蛋白基因GmSRC2的克隆7
2.3融合表达载体pSAT1nEYEPC1和pSAT1cEYEPC1的构建8
3讨论 10
致谢12
参考文献13
附录1基因克隆所需引物列表14
附录2 BiFC载体构建所需引物列表14
效应因子PsAvh23和大豆蛋白GmSRC2的BiFC载体的构建
摘要
大豆是一种重要的农业作物。但是多种病原菌引起的大豆疾病，给世界大豆生产带来经济损失。卵菌疫霉属是其中一种，它能够引起大豆根腐病。病原菌通过Ⅲ型分泌系统分泌的大量效应因子在病原菌侵染植物的过程中可能起到关键性作用，效应因子破坏植物细胞的生理功能和干扰植物细胞正常的生理代谢以破坏植物的防御系统。而经过长期的进化，植物能够释放抗性蛋白与病原菌分泌的效应因子相互识别，引发更为强烈的防御反应。效应因子PsAvh23是大豆疫霉在其侵染过程中分泌的重要效应因子，协调冷蛋白GmSRC2是在大豆中发现的可能与效应因子PsAvh23互作的蛋白，本研究将利用同源重组法构建效应因子PsAvh23和蛋白GmSRC2的BIFC载体，为后续继续验证两者的互作做好准备。
THE CONSTRUCTION OF BiFC VECTORS OF EFFECTOR PsAvh23 AND PROTEIN GmSRC2
ABSTRACT
Soybean is important for agricultural production all over the world. However, the plant diseases induced by several  *51今日免费论文网|www.51jrft.com +Q: #351916072# 
kinds of pathogenic bacteria bring enormous economic loss to global soybean production. One class of filamentous pathogenic bacteria is Oomycetes. Oomycetes can infect plants and animals. And there are a mass of destructive pathogen belongs to this class, such as Phytophthora sojae. Phytophthora sojae can induce stem rot of soybean. Effectors may play an important role during the process that pathogenic bacteria infect host plants. Normally, effectors are targeted in the immunity system of host plants. Effectors are excreted by pathogenic bacteria via T3SS to the plant cell or the apoplast. In order to impair the immunity system of host plants, effectors destroy their physiological function and metabolic activity. In the meantime, plants get the more complex and more effective defensive system to fight against several pathogenic bacteria during the longterm evolution. They can excrete resistant protein that can recognize and interact with effectors. Then, the more intensive defensive reaction is caused. Effector PsAvh23 is excreted by soybean Phytophthora sojea during the process of infecting soybean. And effector PsAvh23 helps a lot in this course of infection. Protein GmSRC2 that is found in soybean may interact with effector PsAvh23. In this research, we construct BiFC vectors of effector PsAvh23 and protein GmSRC2 by means of homologous recombination. And these two vectors will be used in the following research to verify the interaction of effector PsAvh23 and protein GmSRC2.
Key words: Soybean；Phytophthora sojae；Effectors；Immunity Systems; Vector Construction

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