目录
摘要Ⅰ
关键词Ⅰ
AbstractⅡ
引言
引言1
1 材料与方法1
1.1 材料1
1.2 大豆种质资源种子耐涝性鉴定3
1.3 GmWRKY基因家族生物信息学分析 3
2 结果与分析3
2.1 东北大豆种质资源种子耐涝性鉴定3
2.2 耐淹大豆种质的遴选5
2.3 GmWRKY生物信息学分析 6
3 讨论9
3.1 大豆耐涝性鉴定技术有待进一步改进9
3.2 东北大豆耐涝种质的发掘9
3.3 WRKY家族在植物逆境应答中的重要作用 9
3.4 GmWRKY41参与耐涝性调控 10
致谢10
参考文献11
中国东北大豆种质资源发芽期耐涝性鉴定
及GmWRKY41生物信息学分析
摘 要
大豆优异耐涝种质资源的发掘是进行大豆耐涝性育种的基础工作。本研究抽取265份来自东北的大豆种质资源，以相对发芽率为主要指标，结合浸种液电导率和相对苗长，在发芽期对大豆进行耐涝性鉴定，筛选出黑秣食豆、小粒黑豆、延庆茶豆、小黑豆（鉴定统编号H1611）、槐豆（鉴定统编号H1343）等17个耐涝材料。进一步对耐涝和不耐涝大豆转录组分析中的差异表达基因Glyma.05G215900、Glyma.08G021900（WRKY41）基因进行生物信息学分析，结果表明，Glyma.05G215900基因编码363个氨基酸，主要在叶和根中进行表达，在种子、豆荚和花中表达较少；Glyma.08G0219000编码359个氨基酸，主要在豆荚、花和叶中进行表达，在根和种子中表达量较少；进化树显示其与Glyma.01G22480、AT4G23810和AT4G11070亲缘关系较近；Glyma.05G215900和Glyma.08G021900编码的蛋白质均无信号肽及跨膜区；顺式作用元件分析显示Glyma.05G215900和Glyma.08G021900均存在多个逆境胁迫相关作用元件。
IDENTIFICATION OF WATERL *51今日免费论文网|www.51jrft.com +Q: &351916072& 
OGGING TOLETANCE AT GERMINATION STAGE IN SOYBEAN LINES FROM NORTHEAST CHINA AND BIOINFORMATIC ANALYSIS OF GmWRKY41
ABSTRACT
The identification of the elite soybean germplasm resources with waterlogging tolerance is the basis work for breeding purpose. In this study, 265 soybean accessions from Northeast China were selected to evaluate their tolerance to simulated flooding stress at the germination stage, and the relative germination rate was used as the main indicator, combined with the conductivity of the seed soaking solution and the relative whole seedling length.It showed thatseventeen genotyps including Heimoshidou, Xiaoliheidou, Yanqingchadou, Xiaoheidou (identification code is H1611),Huaidou (identification code is H1343), were selected. Further bioinformatics analysis of differentially expressed genes Glyma.05G215900 and Glyma.08G021900 (WRKY41) in transcriptome analysis of waterlogging tolerant and nonwaterlogging tolerant soybean was carried out. The results showed that the Glyma.05G215900 gene encoded 363 amino acids and expressed mainly in leaf and root tissues, while Glyma.08G0219000 gene encoded 359 amino acids and expressed mainly in pod, flower and leaf tissues. The evolutionary tree showed that genetic relationship among Glyma.05G215900, Glyma.08G0219000, Glyma.01G22480, AT4G23810 and AT4G11070 was closely. The proteins encoded by Glyma.05G215900 and Glyma.08G021900 had no signal peptide and transmembrane domain. Cisacting element analysis showed that there were multiple stressrelated elements in both Glyma.05G215900 and Glyma.08G021900 genes.

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