目录
摘要Ⅱ
关键词Ⅱ
AbstractⅢ
引言
文献综述1
引言2
1材料与方法2
1.1材料 2
1.2 测定项目与方法3
1.2.1分离筛选菊芋内生固氮菌3
1.2.2检测菊芋内生固氮菌的固氮酶活性3
1.2.3检测菊芋内生固氮菌的IAA分泌特性及解磷活性3
1.2.4鉴定菊芋内生固氮菌4
1.2.5内生固氮菌的回植 4
1.3数据分析 4
2结果与分析5
2.1筛选获得的菊芋内生固氮菌5
2.2筛选出的菊芋内生固氮菌的种属特性6
2.2.1固氮酶活性6
2.2.2解磷活性6
2.2.3 IAA分泌特性7
2.2.4接种内生固氮菌对菊芋的促生作用8
3讨论9
致谢10
参考文献10
菊芋固氮的促生机制研究
摘 要
菊芋是一种有很强的生态适应性的菊科植物。我们发现菊芋的块茎是一个富含的菊糖的部位，菊糖有降低血糖的作用，也可以当作原料用来生产乙醇。菊芋的内生固氮菌生长在植物的细胞间隙中间，和菊芋本身具有联合固氮的作用。内生固氮菌利用分泌植物生长激素和其他生理活性物质与致病细菌竞争营养和空间的方式来使植物的生长变快。它为将来的进一步研究和应用奠定了基础，也可以为开发高质量和环保的生物细菌肥料提供细菌资源。因此，本实验通过筛选菊芋的内生固氮菌，研究固氮菌在体内的定殖，固氮能力效果等方面的测定。我们从切割下来的菊芋的块茎中分离出来了21株内生固氮菌，其中有10株菌具有解磷活性，解磷活性最高的为Stenotrophomonas sp.，达到177.02ug/ml；10株菌具有IAA分泌活性，IAA分泌活性最高的为Rhizobium sp.，达到33.29ug/ml。另外固氮酶活性最高的菌株为Stenotrophomonas sp.，乙炔还原法检测其活性，能够达到为36.98 nmol/(hmL)。还具有较高的分泌IAA以及活化无机磷的特性。我们从21中菌株中挑选了Chrys *51今日免费论文网|www.51jrft.com +Q: @351916072@ 
eobacterium sp.、Stenotrophomonas sp.、Pseudomonas sp.这3种表现较好的菌株进行温室回植实验，得出在用低氮营养液回植的菊芋中，Chryseobacterium sp.、Stenotrophomonas sp.的生物量都比不接种菌株的高，Pseudomonas sp.的生物量略低于不接种菌株的菊芋。在用中氮营养液回植的菊芋中，不接种菌株的菊芋的生物量高于加了Chryseobacterium sp.、Stenotrophomonas sp.、Pseudomonas sp.的菊芋。不施加氮素的情况下，Stenotrophomonas sp.的生物量远远高于其他菌株处理的菊芋，Chryseobacterium sp.的生物量略高于不接种菌株的菊芋，Pseudomonas sp.的生物量略低于不接种菌株的菊芋。
STUDY ON THE PROMOTING GROWTH MECHANISM OF NITROGEN FIXATION OF JERUSALEM ARTICHOKE
ABSTRACT
Jerusalem artichoke has a strong ecological adaptability. Its tubers are rich in inulin, Inulin can lower blood sugar, and can also be used as a highquality raw material for the production of ethanol. The endogenous nitrogenfixing bacteria of Jerusalem artichoke which exists in the intercellular space of plants and Jerusalem artichoke itself have a joint nitrogenfixing effect. Endogenous nitrogenfixing bacteria promote plant growth by secreting plant growth hormone and other physiologically active substances, competing with pathogenic bacteria for nutrition and space. It has laid the foundation for further research and application in the future, and can also provide bacterial resources for the development of highquality and environmentally friendly biological bacterial fertilizers. Therefore, in this experiment, we screened the endogenous nitrogenfixing bacteria of Jerusalem artichoke to study the colonization of nitrogenfixing bacteria and the determination of the effect of nitrogenfixing ability. We isolated 21 endogenous nitrogenfixing bacteria from the tubers of the tidal flat plant Jerusalem artichoke. Among them, 10 strains had phosphateresolving activity, and the highest phosphateresolving activity was Stenotrophomonas sp., which reached 177.02ug / ml; Activity, the highest IAA secretion activity is Rhizobium sp., reaching 33.29ug / ml. In addition, the strain with the highest nitrogenase activity was Stenotrophomonas sp. The activity was detected by acetylene reduction method, which could reach 36.98 nmol / (h • mL). It also has high secretion of IAA and activation of inorganic phosphorus. We selected Chryseobacterium sp., Stenotrophomonas sp., and Pseudomonas sp. from the 21 strains to perform greenhouse replanting experiments. It was concluded that among the Jerusalem artichokes replanted with low nitrogen nutrient solution, The biomass of Chryseobacterium sp. Stenotrophomonas sp. is higher than that of noninoculated strains, and the biomass of Pseudomonas sp. is slightly lower than that of noninoculated strains of Jerusalem artichoke. Among the Jerusalem artichokes replanted with a medium nitrogen nutrient solution, the biomass of the Jerusalem artichoke without inoculation of the strain was higher than that of the Jerusalem artichoke added with Chryseobacterium sp., Stenotrophomonas sp., and Pseudomonas sp. Among the Jerusalem artichokes replanted only with water, the biomass of Stenotrophomonas sp. is much higher than that of other Jerusalem artichokes. The biomass of Chryseobacterium sp. is slightly higher than that of nonbacterial Jerusalem artichoke. The biomass of Pseudomonas sp. is slightly lower than that of nonbacteria Jerusalem artichoke.

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