目录
摘要II
关键词II
AbstractIII
引言
引言（或绪论）1
1材料与方法3
1.1材料 4
1.1.1试验点概况4
1.1.2试验材料与试剂4
1.2试验设计 4
1.3试验方法 5
1.3.1 土壤DNA的提取，高通量测序及分析 5
1.3.2 土壤可培养微生物的分离纯化 5
1.3.3 潜在抑病细菌与可培养细菌的交叉分析 6
1.4技术路线 6
2结果与分析7
2.1对可培养细菌的分析结果7
2.2对可培养细菌和高通量数据的对比分析结果 8
2.3根际拮抗细菌扩增子序列与可培养细菌的生物信息学分析8
2.3.1 根际的潜在抑病细菌 8
2.3.2拮抗细菌与同属可培养细菌的生物信息学分析8
3讨论 8
致谢9
参考文献10
番茄抑病型土壤可培养细菌群落特征研究
摘 要
抑病型土壤是指即使存在一定数量的病原菌，作物依然不发病或者发病不严重的土壤。实验室的长期田间定位试验点结果表明，利用生物有机肥能够有效调控土壤微生物区系，构建了抑病土壤微生物区系。本文进一步从生物有机肥和化肥长期施用土壤微生物，培育的番茄根际中分离获得纯培养菌株，并结合高通量数据分析的根际微生物群落特征，分析抑病型根际土壤的微生物特征。结果表明：依照宏培养细菌筛选方法共获得103株细菌菌株，分布在5个门和42个属；其中，有22个可培养细菌属在根际土壤中的相对丰度大于0.01%，而另外有17个属的相对丰度小于0.01%，除此之外，有3个属的可培养细菌并未在高通量数据中出现；对高通量数据的分析发现有6个OTUs与抑病相关；进一步结合可培养微生物和高通量数据进行分析发现，4个属的细菌与高通量数据分析得到的6个关键OTUs中的4个OTUs所在的细菌属一致，这为将来对根际关键微生物的验证提供了基础。本研究预期会为抑病型土壤的研究提供新的思路。
Study on the Characterist *51今日免费论文网|www.51jrft.com +Q: ^351916072# 
ics of Culturable Bacterial Community in Tomato Suppressive Soil
ABSTRACT
Suppressive soil refers to the soil that crops would not get disease or not be serious even if there is pathogen in soil. The longterm field experiment of our laboratory showed that the application of bioorganic fertilizer can effectively manipulate the soil microbial flora and construct a suppressive soil. In this study, culturable strains were isolated from the rhizosphere of tomato that sampled from treatments that longterm application of bioorganic fertilizer and chemical fertilizer, and then we analyzed the bacterial characteristics of suppressive rhizosphere combined with highthroughput and isolates data. The results showed that a total of 103 bacterial strains were isolated through the metaculture method, distributed in 5 phylums and 42 genera. Among them, the relative abundance of 22 cultivable bacterial genera in rhizosphere soil were more than 0.01%, while there were 17 genera with a relative abundance of less than 0.01%, and in addition, there were 3 genera of culturable bacteria that did not appear in the highthroughput data; analysis of the highthroughput data found that 6 OTUs were correlated with disease suppression; further analysis based on culturable bacteria and highthroughput data found that the bacteria of the 4 genera are consistent with the bacterial genera of 4 of the 6 key OTUs obtained from the analysis of the highthroughput data. The results of this study could be the base of future verification experiment of the key taxa. This study is expected to provide new ideas for the study of suppressive soils.

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