目录
摘要Ⅱ
关键词Ⅱ
AbstractⅢ
引言
引言1
1□材料与方法2
1.1□材料 2
1.2□方法 2
1.2.1□根系分泌物提取3
1.2.2□根系分泌物与高氯酸盐的结合3
1.2.3□植物吸收3
2□结果与分析3
2.1□根系分泌物与高氯酸盐的定量化4
2.1.1 高氯酸盐与根系分泌物的荧光光谱效应4
2.1.2 SterneVolmer分析5
2.2□植物吸收7
2.2.1 吸收7
2.2.2 降解10
2.3□结论10
3□讨论11
3.1□根系分泌物与高氯酸盐的相互作用11
3.3□根系分泌物对植物吸收高氯酸盐的影响11
3.2□根际微环境中其它要素与高氯酸盐的相互作用11
3.4□研究展望12
3.5□实验过程中可能产生的偏差12致谢16
参考文献17
根系分泌物对植物吸收高氯酸盐的影响
摘 要
环境中的高氯酸盐作为一种新型污染物，经植物吸收后可在其体内累积，对农产品的生产安全产生重要的影响，但植物吸收高氯酸盐的关键因子和机制尚未明确，值得深入探索。植物根部是环境中高氯酸盐进入植物体内的主要通道，根系分泌物可能会与根际微环境中的高氯酸盐产生相互作用，从而影响植物根系对高氯酸盐的吸收。因此，本实验通过在水培植物中添加提取出的根系分泌物，并置于10mg/L的NaCOl4中暴露9天，与未添加根系分泌物的对照组相比，发现在培养基中添加根系分泌物会在初始暴露阶段加速植株对高氯酸盐的吸收；但到暴露中期，吸收速度放缓；最终高氯酸盐积累量较对照组少，而ClO2、Cl积累含量显著高于对照组。本实验还通过微量荧光滴定技术来研究根系分泌物与高氯酸盐的相互作用关系。结果表明，在pH为3、5、9的条件下，根系分泌物与高氯酸盐相互结合。这可能是导致植物根部总体吸收高氯酸盐减少的原因。
ABSTRACT
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pollutant, perchlorates in the environment can be absorbed and accumulated in plants, which has a great impact on the safty of agricultural products, but the absorption mechanism of plants and the key to affecting the absorption of perchlorates are not yet clear and are still worth exploring in depth. The root of the plant is the main channel for the perchlorates in the environment to enter the plant body. Root exudates may interact with the perchlorates in the rhizosphere microenvironment, thereby affecting the absorption of perchlorates by the plant root. Therefore, in this experiment, by adding the extracted root exudates to hydroponic plants and placing them in 10 mg/L NaC1O4 for 9 days, compared with the control group without adding root exudates. It was found that the roots exudates can accelerate the absorption of perchlorates by the plants in the initial exposure stage; but by the middle of the exposure, the absorption rate was slowing down; eventually the amount of perchlorates accumulation is less than the control group, and the ClO2, Cl accumulation content is significantly higher than the Control group. In this experiment, the microfluorescence titration technique was also used to explore the interaction between root exudates and perchlorates. The results showed that under the conditions of pH 3, 5, 9 ,the root exudates would combine with perchlorates, which may be the cause of the overall reduction in perchlorates absorption by plant roots.

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