目录
摘要I
关键词I
AbstractII
引言
引言1
1材料与方法3
1.1 实验材料 3
1.1.1 植物材料及实验设计 3
1.1.2 数据处理网站及软件 3
1.2 研究方法 3
1.2.1实验材料的处理3
1.2.2收集数据4
1.2.3分析并整理数据4
1.2.3作图比较4
2 结果与分析4
2.1 蛋白质的表达差异概述4
2.2 KEGG（代谢途径）分析5
2.3 BP（生物学过程）分析7
2.3胁迫响应蛋白的含量对比12
3 讨论12
致谢13
参考文献 14
硫氢化钠缓解水稻干旱胁迫的蛋白质组学分析
摘 要
干旱作为影响中国农业生产的重要非生物因素，也大幅影响着水稻的产量，因此研究水稻抗旱是保证农业发展的重要举措之一。硫化氢是第三种气体信号分子，其在调节植物生长发育、增强植物生物和非生物抗逆性等方面有重要作用，但其在水稻抗旱性方面研究尚不明确。为了明确硫化氢在水稻干旱胁迫中的作用机制，本实验以硫氢化钠作为硫化氢供体，利用蛋白质组学技术，研究了干旱胁迫下水稻的差异蛋白比较并对这些蛋白做基因本体（Gene Ontology，GO）、京都基因与基因组百科全书（Kyoto Encyclopedia of Genes and Genomes，KEGG）分析，探讨他们在生物学过程和代谢途径中的不同。研究发现在硫氢化钠预处理后的干旱胁迫组水稻中的部分蛋白，如非特异性脂质转移蛋白、硫氧还蛋白、脂肪氧化酶等的含量都较干旱处理组有上调，暗示了硫氢化钠处理后的水稻在受到干旱胁迫时，通过调控胁迫响应相关蛋白的浓度来达到缓解的效果，为进一步研究硫化氢在水稻抗旱性方面的应用打下基础。
PROTEOMIC ANALYSIS OF SODIUM HYDROSULFIDE IN ALLEVIATING DROUGHT STRESS IN ORYZA SATIVA
ABSTRACT< *51今日免费论文网|www.jxszl.com +Q: @351916072@ 
br /> Drought, as an important abiotic factor affecting agricultural production in China, also has a significant impact on rice yield. Therefore, research on rice drought resistance is one of the important measures to ensure agricultural development. Hydrogen sulfide is the third gas signal molecule, which plays an important role in regulating plant growth and development, enhancing plant biotic and resistance, but its research on rice drought resistance is not clear. In order to clarify the mechanism of hydrogen sulfide in Rice under drought stress, this experiment used sodium hydrosulfide as hydrogen sulfide donor, and used proteomics technology to study the differences of rice proteins under drought stress, and to do Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis of these proteins, to explore their differences in biological process and metabolic pathway. It was found that the contents of some proteins, such as nonspecific lipid transfer protein, thioredoxin and lipoxygenase, were upregulated in the drought stress group of rice pretreated with sodium hydrosulfide, which suggested that the rice pretreated with sodium hydrosulfide could alleviate the drought stress by regulating the concentration of stress response related proteins The application of hydrogen sulfide in rice drought resistance was studied.

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