目录
摘要I
关键词I
AbstractI
引言
引言
1 材料与方法1
1.1植物材料与生长条件1
1.2 菌株、质粒和试剂盒 1
1.3突变体外源激素处理1
1.4 GIW1蛋白结构域分析2
1.5同源蛋白系统进化树分析2
1.6亚细胞定位分析2
1.6.1目的蛋白在水稻原生质体细胞中的表达2
1.6.1.1 GIW1PAN580重组载体的构建2
1.6.1.2水稻原生质体转化2
1.6.2目的蛋白在烟草叶片细胞中的表达3
1.6.2.1 GIW1GFP重组载体的构建3
1.6.2.2冻融法转化农杆菌3
1.6.2.3农杆菌介导烟草瞬时转化3
1.7蛋白原核表达4
1.8 GIW1基因过表达载体的构建4
2 结果与分析5
2.1 GIW1蛋白结构域分析5
2.2 GIW1及其同源蛋白进化分析5
2.3 GIW1基因GO（gene ontology）分析 6
2.4 GIW1基因表达相关性分析7
2.5 GIW1基因表达模式分析10
2.6 GIW1蛋白亚细胞定位11
2.7 GIW1蛋白原核表达12
2.8 giw1基因突变体表型分析13
2.9 GIW1突变体对BR的响应13
2.10 GIW1过表达载体的构建14
3讨论 15
致谢16
参考文献16
GIW1调控水稻粒长的分子机制研究
摘要
油菜素甾醇类化合物(brassinosteroids, BRs)调节水稻株高、分蘖、叶夹角和籽粒大小等重要的农艺性状，然而其潜在的作用机制仍不清楚。本实验室前期研究发现水稻粒长基因qGL3负调控粒长和BR信号途径，本研究继续深入分析qGL3调控BR信号的分子机制。qGL3可以与一个含有WD40结构域的蛋白相互作用，将其命名为GIW *51今日免费论文网|www.jxszl.com +Q: &351916072& 
1 (qGL3interacting WD40 protein 1)，GIW1可能通过调控BR信号途径影响水稻籽粒大小。本文通过研究GIW1GFP在水稻原生质体细胞、烟草叶片细胞中的定位情况，以及预测GIW1基因在水稻不同组织、不同发育时期的表达情况，探究GIW1的生物学功能。获得了GIW1的TDNA插入突变体材料，giw1突变体表现出叶夹角变大，籽粒变长的表型。对giw1突变体材料种子进行BL激素处理，发现突变体对BL更加敏感，GIW1可能是BR信号的负调控因子。
FUNCTIONAL ANALYSIS OF GIW1 INVOLVED IN GRAIN LENGTH REGULATION IN RICE(Oryza sativa L.)
ABSTRACT
Brassinosteroids (BRs) regulate plant height, tillering, leaf angle, grain size and other important agronomic traits in rice. However, the underlying mechanism is still unclear. Previous studies in our laboratory found that the rice grain length gene qGL3 negatively regulated the grain length and BR signal pathway. In this study, we continue to analyze the molecular mechanism of qGL3 regulating BR signal. qGL3 can interact with a protein containing WD40 domain, named GIW1 (qGL3 interacting WD40 protein 1). GIW1 may affect rice grain size by regulating br signal pathway. In this paper, we studied the location of GIW1GFP in rice protoplast cells and tobacco leaf cells, and predicted the expression of GIW1 gene in different tissues and development stages of rice to explore the biological function of GIW1. Furthermore, the TDNA insertion mutant material of GIW1 was obtained. The mutant of giw1 showed the phenotype that the leaf angle became larger and the grain became longer. In this study, we will treat the seeds of giw1 mutant with BL hormone, and find that the mutant is more sensitive to BL, and GIW1 may be a negative regulator of BR signal.

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