2临床上病毒性疾病给宠物的健康造成了巨大的威胁，纳米银作为一种非常具有潜力的抗病毒药备受关注，但纳米银的生物安全性也成为人们关注的焦点。本研究利用没食子酸作为还原剂和稳定剂绿色合成纳米银并进行了表征分析，通过体外细胞培养方法评价了其对猫肾细胞的细胞毒性作用。对纳米银的理化性质表征分析，透射电子显微镜结果显示纳米银粒子的平均粒径为13.81 ± 2.21 nm；紫外可见光谱最大吸收波长在400.5 nm处；水动力尺寸为21.35 ± 0.90 nm；zeta电位结果为-38.6 ± 0.91 mV，表明纳米银粒径均一、无聚集。MTT法检测猫肾细胞的细胞毒性结果显示，细胞的存活率随着纳米银浓度的降低而升高，且纳米银浓度在0.25~8 μg/mL范围内时，细胞存活率达到80%以上，表明纳米银的细胞毒性很小。综上所述，没食子酸绿色还原法制备的纳米银粒子粒径均匀，纯度较高，分散性较好，低浓度下具有很小的细胞毒性，在兽医临床中具有一定的应用前景。
目 录
Abstract2
Key words2
引言2
1材料与方法4
1.1 材料4
1.1.1细胞4
1.1.2试剂4
1.1.3试验仪器4
1.2 纳米银溶液的制备4
1.3 纳米银溶液的理化性质表征4
1.3.1粒径测定4
1.3.2 zeta电位测定4
1.3.3 透射电子显微镜分析4
1.3.4 紫外可见吸收光谱分析4
1.3.5 红外线光谱分析4
1.4 细胞毒性的测定4
2结果与分析5
2.1 纳米银溶液的性质测定的结果5
2.2 纳米银溶液的理化性质表征结果6
2.2.1 粒径测定结果6
2.2.2 zeta测定结果6
2.2.3 透射电子显微镜分析结果6
2.2.4 紫外可见吸收光谱分析结果7
2.2.5 红外线光谱分析结果7
2.3 细胞毒性测定结果8 讨论8
致谢9
参考文献10
纳米银的制备、表征及其细胞毒性的 *景先生毕设|www.jxszl.com +Q: *351916072* 
研究
动物医学专业学生 刘国彦
指导教师 郭大伟
Preparation , Characterization and evaluation of cell cytotoxicity of silver nanoparticles
Student majoring in Verinary Medicine Liu Guoyan
Tutor Guo Dawei
Abstract：Clinical viral disease caused a huge threat to the health of the pet , and silver nanoparticles has received much attention as a very promising antiviral drug. But, the safety of silver nanoparticles has been the focus of attention. In this study, gallonic acid was used as a reducing agent and stabilizer to synthesize nano silver and characterized by characterization. Besides, the cytotoxicity of cat kidney cells was evaluated by cell culture in vitro. The physichemical properties of AgNPs were further characterized. The image of transmission electron microscopy (TEM) showed that SNPs exhibited approximately spherical shape with the average diameter of 13.81 ± 2.21 nm. The absorbance peak of obtained SNPs was sharp with the maximum wavelength of 400.5 nm by ultravioletvisible (UVvis) spectroscopy. Their hydrodynamic diameters were 21.35 ± 0.90 nm , and zeta potentials were 38.6 ± 0.91 mV, respectively. These data suggested that AgNPs had the good dispersivity in the solution and the particle size is uniform. MTT assay showed that the survival rate of cells increased with the decrease of nanosilver concentration, and the cell viability was over 80% when the concentration of nanosilver was in the range of 0.25 ~ 8 μg / mL. Indicating that the cytotoxicity of nanosilver is very small. In summary, the silver nanoparticles prepared by the green reduction method of gallic acid have uniform particle size, high purity, good dispersibility and low cytotoxicity at low concentration to cat kidney cells, which has a certain application prospects In the veterinary clinic.

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