1
目 录
Abstract1
Key words1
引言1
1 材料与方法3
1.1 材料3
1.1.1 采血及血清样品处理3
1.1.2 主要试剂3
1.1.3 主要仪器3
1.2 方法3
1.2.1 血清样品制备4
1.2.2 洗涤溶液制备4
1.2.3 CSF操作步骤4
1．2．4 PRRSV检测4
1．2．5 结果判定4
1．2．5．1 CSFV结果判定4
1．2．5．2 PRRSV结果判定4
2 结果与分析4
2.1 猪瘟抗体检测结果分析4
2.2 猪繁殖与呼吸综合征抗体检测结果分析5
3 讨论6
3.1 猪瘟结果讨论6
3.2 猪繁殖与呼吸综合征检测结果讨论6
致谢7
参考文献8
猪瘟病毒和猪繁殖与呼吸综合征病毒的ELISA检测
动物医学专业学生 高启松
指导教师 雷治海
摘要：猪瘟是以稽留高热、皮肤和黏膜出现大量出血点为主要特征的一种接触性传染病。世界卫生组织（OIE）将猪瘟列入A类传染病。猪繁殖与呼吸综合征是由猪繁殖与呼吸综合征病毒引起，以母猪呼吸道疾病及流产、死胎、木乃伊胎等繁殖障碍和仔猪呼吸障碍、死亡率高等为特征的急性高度接触性传染病。这两种疾病对全球范围内的畜牧业危害极大，造成严重的经济损失。为了了解江苏省某规模化猪场的免疫状况，实时监测其群体免疫状况，及时发现存在的问题及潜在风险。本文采用间接ELISA检测方法对205份猪血清样品进行猪瘟病毒和猪繁殖与呼吸综合征病毒检测。结果表明，猪瘟病毒抗体检出阳性120份，检出率69.3%，猪繁殖与呼吸综合征病毒抗体检出阳性192份，检出率93.6%。
Elisa Test For Swine Fever Virus and Porcine Reproductive and Respiratory Syndrome Virus
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Student majoring in Veterinary Medicine GAO Qisong
Tutor LEI Zhihai
Abstract： Swine fever is a contagious disease characterized by continued high fever and a large number of hemorrhagic points in the skin and mucosa. The World Health Organization (OIE) ranks swine fever into Class A infectious diseases. Porcine reproductive and respiratory syndrome is caused by porcine reproductive and respiratory syndrome virus, which is an acute, highly contagious disease characterized by reproductive disorders such as the respiratory diseases of the sows and the miscarriage, stillbirth, mummification, etc and piglets breathing disorder, mortality rate is high. The two diseases are extremely harmful to animal husbandry in the world, causing serious economic damage. In order to understand the immune status of a largescale pig farm in Jiangsu province, the immune status of the group was monitored in real time, and the problems and potential risks were detected in time. In this paper, 205 samples of porcine serum samples were tested by swine fever virus and porcine reproductive and respiratory syndrome virus by indirect ELISA. The results showed that the positive number of swine fever virus antibody was 120, the detection rate was 69.3%, and the positive number of porcine reproductive and respiratory syndrome virus was 192, the detection rate was 93.6%.
Key words: Swine fever virus；Porcine reproductive and respiratory syndrome virus；ELISA；

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