1猪呼吸道疾病是由细菌、病毒或寄生虫单独和/或混合感染引起的猪肺炎、支气管炎等疾病的通称，是一种世界范围内危害养猪业健康发展的重要疾病。该病病因复杂，病原众多，易受到转群运输、极端天气、室内温湿度、饲养密度、代谢紊乱、免疫低下等因素影响，具有发病率高、死亡率高、饲料报酬率低和经济损失严重等特点。若治疗不及时，将影响猪的生长发育，导致饲料利用率低，甚至死亡，给猪场带来严重损失。针对该病，目前国内外尚缺乏有效的疫苗预防，仍主要依靠抗菌药物防治。本文以加米霉素为研究对象，采用微量肉汤稀释法测定其对猪呼吸道常见致病细菌的最小抑菌浓度。同时，通过与对照药物替米考星相比较，进一步佐证其抑菌效果，从而为临床合理应用加米霉素提供依据。
目 录
Abstract 1
Key words 1
引言 1
1材料与方法 3
1.1材料 3
1.1.1 药品 3
1.1.2 试验菌株 3
1.1.3 培养基和试剂 3
1.1.4 主要仪器和设备 3
1.1.5 试验时间地点 4
1.2 试验方法 4
1.2.1 药品储备液的制备 4
1.2.2 菌液的制备 4
1.2.3 药敏试验的操作 4
1.2.4 药敏试验质控 4
1.2.5 结果判定 4
1.3 数据处理 5
2结果与分析 5
3小结 9
致谢 9
参考文献 9
加米霉素对呼吸道常见病原菌的体外抗菌活性研究
动物药学专业学生 范琪
指导教师 余祖功
In vitro antibacterial activity of common pathogens isolated from porcine respiratory tract of Gamithromycin
Student majoring in animal pharmacy Qi Fan
Tutor Zugong Yu
Abstract：Porci *景先生毕设|www.jxszl.com +Q: ^351916072# 
ne respiratory disease is caused by bacteria, viruses or parasites alone and / or mixed infection caused by porcine pneumonia, bronchitis and other diseases known as a worldwide threat to the healthy development of the pig industry, an important disease. The cause of the disease complex, many pathogens. It is easy to be influenced by such factors as trans group transportation, extreme weather, indoor temperature and humidity, feeding density, metabolic disorder, immunocompromised and so on. It has the characteristics of high morbidity, high mortality, low feed rate and serious economic losses. If the treatment is not timely, it will affect the growth and development of pigs, leading to low utilization of feed and even death, which will cause serious losses to the pig farms. In view of this disease, there is still a lack of effective vaccine prevention at home and abroad, and it still relies mainly on the prevention and control of antimicrobial agents. This paper intends to use the Gamithromycin for the object, which is the novel semisynthesis macrolide for animals.Then,The minimum inhibitory concentration of common pathogenic bacteria in porcine respiratory tract was determined by microdilution broth dilution method.At the same time,compared with the control drug tilmicosin,further evidence of its antibacterial effect, so as to provide the basis for the clinical application of Gamithromycin.
Key words: Porcine respiratory disease；Gamithromycin；Minimum inhibitory concentration；Microdilution broth dilution method;
引言
猪呼吸道疾病是目前我国规模化猪场的常发病、多发病，一年四季均有发生，尤其是在每年的春、秋季节，由于气温骤变、猪舍潮湿、通风不良、猪群饲养密度过大时最易发生该病，有的猪场发病率高达40%～80%，其中直接死亡率可达20%[1]。其危害性极大，不但造成猪的直接死亡，而且猪群增重缓慢,出栏延迟，已成为养猪疫病防治中最突出和难控制的问题[2]。若防治与治疗不到位，很容易给养猪业造成重大损失[3]。
引起猪呼吸系统疾病的病原菌非常多[12]，其中病毒病原有猪繁殖与呼吸综合征病毒(PRRSV)、圆环病毒2 型(PCV2)、伪狂犬病病毒(PRV)、流感病毒(SIV)、猪瘟病毒(HCV)等。细菌病原有支气管波氏杆菌、放线杆菌、多杀性巴氏杆菌、副猪嗜血杆菌、链球菌等。此外还有支原体、附红细胞体及弓形体等。猪群受到上述病原的感染后，会使得猪的机体免疫力下降，给其它病原体的侵入提供了条件。同时也易产生十分严重的继发性感染，使呼吸系统疾病的病情更严重,导致猪群死亡率升高[3]。

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