目录
摘要III
关键词III
Abstract IV
引言
1选题背景1
1.1课题来源以及课题目的和意义1
1.2国内外研究状况1
1.3本实验理论目的要求1
2文献综述1
2.1鸡油菌简介1
2.2鸡油菌多糖的分离纯化2
2.2.1分离2
2.2.2纯化2
2.3鸡油菌多糖的组成及结构解析2
2.3.1水解3
2.3.2高碘酸氧化和smith降解法3
2.3.3红外光谱法3
2.3.4核磁共振法3
2.3.5色谱法3
2.4鸡油菌多糖的活性研究4
2.4.1抗氧化性4
2.4.2降血糖作用4
2.4.3免疫调节作用4
2.4.4体外肿瘤抑制作用研究5
2.4.5体内肿瘤抑制作用研究5
3实验材料、仪器与方法5
3.1鸡油菌多糖的分离纯化5
3.1.1.材料与仪器5
3.1.2实验方法6
3.1.2.1分离6
3.1.2.2纯化6
3.2鸡油菌多糖结构鉴定7
3.2.1材料及仪器7
3.2.2实验方法7
3.2.2.1鸡油菌多糖含量测定7
3.2.2.2鸡油菌多糖的单糖组成分析8
3.2.2.3鸡油菌多糖的分子量测定8
3.2.2.4鸡油菌多糖的结构解析9
3.3鸡油菌多糖对肠道菌群的调节9
3.3.1材料与仪器9
3.3.2实验方法9
4结果与分析10
4.1鸡油菌多糖的分离纯化10
4.1.1分离10
4.1.2纯化10
4.2鸡油菌多糖的结构鉴定10
4.2.1鸡油菌多糖总糖含量测定10
4.2.2鸡油菌多糖单糖组成分析11
4.2.3高效液相色谱法测鸡油菌多糖分子量12 *51今日免费论文网|www.jxszl.com +Q: #351916072# 

4.2.4核磁共振法解析鸡油菌多糖结构13
4.3鸡油菌多糖对肠道微生物菌群的调节13
5讨论与展望13
5.1分离纯化13
5.2结构鉴定14
5.3活性评价14
5.4展望14
致谢14
参考文献15
鸡油菌多糖的分离纯化、结构鉴定与活性研究
摘 要
鸡油菌是我国四大名菌之，富含维生素C、胡萝卜素、蛋白质、钙、磷、铁等。其可食用、药用，味性甘、寒，具有清目、益肠、利肺的功效，鸡油菌提取物的抗氧化性、抗癌性、降血糖性已经得到了初步的证实。本实验旨在对鸡油菌多糖进行分离纯化与结构鉴定，之后再评价其对肠道菌群调节作用。采用热水浸提法提取粗多糖；离子交换柱进行纯化；高效液相色谱法测分子量和单糖组成；核磁共振法解析其高级结构；研究鸡油菌多糖对肠道菌群的调节。通过实验，主要了解鸡油菌多糖的结构以及其对生物肠道菌群的调节作用，为后来者研究鸡油菌多糖提供一定理论基础。
SEPARATION AND PRIFICATION, STRUCTURE IDENTIFICATION AND ACTIVITY STUDY OF POLYSACCHARIDES FROM CANTHARELLUS CIBARIUS
ABSTRACT
Cantharellus cibarius is one of the four famous fungus in China. Cantharellus cibarius is rich in Vc, carotene, protein, calcium, phosphorus, iron and so on. Cantharellus cibariuse is edible and medicinal, with sweet and cold taste, clear eyes, benefit intestines and benefit lungs. The antioxidant, anticancer and hypoglycemic properties of cantharellus cibarius extract have been preliminarily confirmed. The aim of this study was to isolate, purify and identify the structure of the polysaccharides from Coprinus Comatus, and then to evaluate its regulation effect on Intestinal Flora. The crude polysaccharide was extracted by hot water extraction, purified by Ion Exchange column, Molecular Weight and monosaccharide composition were determined by Highperformance liquid chromatography, and its high structure was analyzed by nuclear magnetic resonance method. Through the experiment, we mainly understand the structure and the regulation of the Microbial Flora in the intestinal tract of the chicken oil fungus polysaccharide, which provides a theoretical basis for the later research.

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